Tularemia in a common marmoset (Callithrix jacchus) diagnosed by 16S rRNA sequencing

Veterinary Microbiology
H PosthausP Kuhnert

Abstract

We report a case of tularemia in a common marmoset (Callithrix jacchus) diagnosed by determination of the isolate's 16S ribosomal RNA (rRNA) gene sequence. Pathological examination of the animal revealed a multifocal acute necrotizing hepatitis, interstitial nephritis, splenitis, and lymphangitis of the mandibular, retropharyngeal, and cervical and mesenteric lymph nodes. Moreover, multiple foci of acute necrosis were found in the epithelium of the jejunum and the interstitium of the lung. Bacteriological investigations revealed a septicemia. The isolated infectious agent was uncommon, not routinely diagnosed in our laboratory and therefore difficult to identify by conventional tools in a reasonable time and effort. thus, we decided to perform a genetic analysis based on the 16S rRNA gene sequence. Thereby, an infection with Francisella tularensis, the causative agent of tularemia, was unambiguously diagnosed. This shows the great advantage 16S rRNA gene sequencing has as a general identification approach for unusual or rare isolates.

Citations

Jul 3, 2009·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Cornelia J Ketz-RileyJeannine M Petersen
Apr 5, 2003·Journal of the American Veterinary Medical Association·Katherine Anne Feldman
Jul 18, 2006·Journal of the American Veterinary Medical Association·Catherine S Beckwith
Apr 2, 2009·International Journal of Experimental Pathology·Michelle NelsonAndrew J H Simpson
May 2, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Miklós GyuraneczKároly Erdélyi
Aug 16, 2018·Frontiers in Cellular and Infection Microbiology·Paola Pilo
Nov 13, 2020·BMC Veterinary Research·Felix GiebelsVeronika Maria Stein

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