Tulbaghia violacea (Harv) Exerts its Antifungal Activity by Reducing Ergosterol Production in Aspergillus flavus.

Current Microbiology
B M SomaiCarminita Frost

Abstract

Opportunistic infections in immunosuppressed patients have led to an increase in fungal infections, with Aspergillus being one of the main causative agents. Medicinal plants exhibiting antifungal activity have the potential to be used as chemotherapeutic agents. However, often their mechanisms of action are not fully researched. Tulbaghia violacea exhibits antifungal activity towards Candida, Aspergillus flavus and Aspergillus parasiticus but its mode of action has only recently begun to be investigated. This study aimed to ascertain the effect of T. violacea rhizome extracts on ergosterol production in A. flavus and the mechanism of inhibition. The MIC of a T. violacea rhizome extract against A. flavus was first determined, using a broth dilution assay, to be 15 mg/ml. Thereafter, the culture was subjected to sub-inhibitory concentrations of the extract before sterol intermediates of the ergosterol biosynthetic pathway were isolated and analysed for dose-dependent accumulation. Analysis by reverse-phase HPLC displayed a decline in ergosterol production in a dose-dependent manner when exposed to increasing concentrations of T. violacea extract. Quantification of the sterol intermediates of the ergosterol pathway indicated a def...Continue Reading

References

Apr 1, 1996·Antimicrobial Agents and Chemotherapy·R C GoldmanJ H Griffin
Jan 30, 2002·Trends in Molecular Medicine·Antonella LupettiSteven Kelly
Jan 15, 2008·Steroids·Laura Alcazar-FuoliJuan L Rodriguez-Tudela
Jan 21, 2009·Drug Discovery Today·Katharina GauwerkyHans C Korting
Feb 22, 2011·Phytochemistry·Jörg M AugustinSøren Bak
Jun 15, 2011·Journal of Food Protection·Benesh Munilal Somai, Vuyokazi Belewa
Apr 27, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E PintoL Salgueiro
Dec 18, 2013·FEMS Microbiology Ecology·Paolina GarbevaWietse de Boer
Jan 31, 2017·Journal of Applied Microbiology·V BelewaB M Somai
Jul 22, 2019·Fungal Genetics and Biology : FG & B·Margriet W J HokkenP E Verweij
Jan 19, 2020·BMC Infectious Diseases·Namareq F AldardeerBasem Alraddadi
Jul 19, 2020·Genes·Tania Jordá, Sergi Puig

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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