Tumor antigen-specific immunization of bone marrow transplantation donors as adoptive therapy against established tumor

Journal of the National Cancer Institute
R L HornungL W Kwak

Abstract

Persistence of the underlying malignancy remains the main obstacle to the successful treatment of human malignancies with high-dose chemoradiotherapy and bone marrow transplantation. The aim of this study was to determine whether antigen-specific antitumor immune responses, elicited in normal donor mice by immunization with the soluble form of a surrogate tumor antigen (i.e., ovalbumin [OVA]), can be transferred via bone marrow transplantation into lethally irradiated, syngeneic recipient mice. An additional goal was to evaluate the ability of these adoptively transferred bone marrow cells to eradicate established recombinant OVA-expressing lymphomas that recurred after lethal-dose total-body irradiation (TBI). Female C57BL/6 donor mice were immunized twice with OVA emulsified in a muramyl-dipeptide-containing adjuvant. Syngeneic mice bearing a day-10 or day-11, approximately 1-cm subcutaneous E.G7-OVA tumor (E.G7-OVA tumor cells were derived from transfection of EL-4 thymoma tumor cells using the coding sequence of chicken OVA gene complementary DNA) were treated with TBI and reconstituted with bone marrow from nonimmune or OVA-immunized mice. In subsequent experiments, tumor-bearing mice, treated with TBI and OVA-immune bone ...Continue Reading

Citations

Dec 11, 2002·Cytotherapy·J G Gribben
Jun 18, 2002·Seminars in Oncology·Francine M Foss
Jul 30, 2004·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·W AsavaroengchaiJ J Mulé
Jun 22, 2005·Bone Marrow Transplantation·S S NeelapuL W Kwak
Jul 19, 2001·Annals of the New York Academy of Sciences·R HandgretingerD Niethammer
Oct 20, 1999·Blood Reviews·G Cook, J D Campbell

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