PMID: 28502299May 16, 2017Paper

Tumor-associated macrophages promote the proliferation and migration as well as invasion of sorafenib-resistant hepatocellular carcinoma cells

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Xufu WeiZhongjun Wu

Abstract

Objective To investigate the roles of tumor-associated macrophages (TAMs) and epithelial growth factor receptor (EGFR)/β-catenin signaling pathway in sorafenib resistance of hepatocellular carcinoma cells. Methods Macrophages were isolated from peripheral blood mononuclear cells (PBMCs) and cultured in the presence of colony-stimulating factor (M-CSF) to obtain TAMs. Phenotypes of TAMs were identified. The level of epithelial growth factor (EGF) secreted by TAMs was detected by ELISA. CCK-8 assay was performed to verify the effects of EGF on HepG2 and SMMC7721 cell proliferation. Transwell(TM) assay was used to examine the effects of EGF on the invasion and migration ability of HepG2 and SMMC7721 cells. TAMs and hepatocellular carcinoma cells were co-cultured to study the downstream signaling pathways. Sorafenib-resistant HepG2 and SMMC7721 strains (R-HepG2 and R-SMMC7721 cells) were prepared and then subjected to Western blotting and immunohistochemistry to examine the expression levels of β-catenin and EGFR. Results TAMs we prepared were confirmed. Compared with HepG2 and SMMC7721 cells, R-HepG2 and R-SMMC7721 showed enhanced proliferation, invasion and migration abilities. The growth rates of sorafenib-resistant cell lines a...Continue Reading

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