Tumor cell invasion of basement membrane in vitro is regulated by amino acids

Cancer Investigation
R K SinghG P Siegal

Abstract

Because most cancer deaths result from disseminated disease, understanding the regulation of tumor invasion and metastasis is a central theme in tumor cell biology. Interactions between extracellular matrices (ECM) and cellular microenvironment play a crucial role in this process. We have tested selected amino acids and polyamines for their ability to regulate RL95-2 cell invasion through both intact human amniotic basement membrane and a novel human ECM (Amgel). Three major systems for neutral amino acid transport, systems L, A, and ASC, are operational in these neoplastic cells. Amino acids entering the cell via transport system A or N, i.e., (methyl amino)-isobutyrate (MeAIB) or Asn, markedly enhanced invasiveness of these human adenocarcinoma cells as measured by a standard 72-hr amnion or Amgel invasion assay. Addition of 2-amino-2-norborane carboxylic acid (BCH; 1 mM), a model substrate of the L transport system, caused a significant decrease in invasive activity when tested in the Amgel assay. Interestingly, Val lowers steady-state levels of MeAIB uptake and blocks the increase in cell invasion elicited by MeAIB. At the same time, these amino acids do not influence cell proliferation activity. Neither the charged amino a...Continue Reading

References

Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·K Y Chen, E S Canellakis
Dec 1, 1990·Cancer Metastasis Reviews·W G Stetler-Stevenson
Nov 25, 1987·Biochimica Et Biophysica Acta·K TryggvasonT Salo
Jan 1, 1987·Annual Review of Nutrition·E J Collarini, D L Oxender
Mar 1, 1987·Journal of Cellular Biochemistry·I B Weinstein
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C A Rinehart, E S Canellakis
Dec 28, 1973·Biochimica Et Biophysica Acta·H N ChristensenG Ronquist
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G P SiegalS L Berger
Apr 15, 1994·The Biochemical Journal·J D McGivan, M Pastor-Anglada
Jan 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M S KilbergR O Laine
Jun 30, 1993·Biochemical and Biophysical Research Communications·T C ChilesM S Kilberg
Jan 1, 1993·Annual Review of Nutrition·M S KilbergD A Novak

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Citations

Jun 17, 2008·Pathology Oncology Research : POR·Kun YuanGene P Siegal
May 4, 2010·PloS One·David Montaner, Joaquín Dopazo
Apr 17, 2008·Journal of Structural Biology·Zhong-Ying ShenLi-Jun Tan
May 15, 2007·American Journal of Physiology. Cell Physiology·Shiho KanekoSeiji Ito
Nov 13, 2001·American Journal of Physiology. Renal Physiology·J ChillarónM Palacín

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