Tumor cell secretion of soluble factor(s) for specific immunosuppression

Scientific Reports
Arihiro Kano

Abstract

Studies of tumor models using syngeneic transplantation have advanced our understanding of tumor immunity, including both immune surveillance and evasion. Murine mammary carcinoma 4T1 cells secrete immunosuppressive soluble factors as demonstrated in splenocyte culture. Cultured primary splenocytes secrete IFN-γ, which was strikingly elevated when the cells were isolated from 4T1 tumor-bearing mice. The secretion of IFN-γ peaked a week after 4T1 inoculation and then declined. This reduction may be due to the relatively decreased lymphocytes and increased granulocytes in a spleen accompanied by splenomegaly with time after the 4T1 inoculation. IFN-γ production was further suppressed with the addition of the conditioned media from 4T1 cells to the splenocyte culture. This suppressive effect was more evident in the splenocytes isolated from mice that had 4T1 tumors for a longer period of time and was not observed in the conditioned medium either from CT26 cells or with splenocytes isolated from CT26 tumor-bearing mice. These results suggest that the IFN-γ suppression is 4T1 tumor-specific. The soluble factor(s) in the 4T1-conditioned media was a protein between 10 to 100 kDa. The cytokine tip assay demonstrated several known cytok...Continue Reading

References

Jan 1, 1993·Annual Review of Immunology·M A Farrar, R D Schreiber
Jul 2, 1998·Clinical and Experimental Immunology·S JodoT Koike
Jul 29, 1998·The Journal of Urology·Y MizutaniB Bonavida
Jul 17, 1999·British Journal of Cancer·M KimuraK Takahashi
Jun 23, 2000·Obstetrics and Gynecology·L HeflerC Tempfer
Feb 15, 2001·Microscopy Research and Technique·C BeckD Rowley
Mar 29, 2001·Nature Immunology·M J SmythJ A Trapani
Oct 31, 2002·Nature Immunology·Gavin P DunnRobert D Schreiber
Oct 31, 2002·Nature Immunology·Hung T Khong, Nicholas P Restifo
Mar 1, 1965·Experimental Cell Research·L HAYFLICK
Nov 19, 2003·The Journal of Experimental Medicine·Arihiro KanoXin-Yuan Fu
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaoyan ShiXiaojing Ma
Aug 17, 2004·Immunity·Gavin P DunnRobert D Schreiber
Jun 20, 2006·Cancer Immunology, Immunotherapy : CII·Chien-Chung Chang, Soldano Ferrone
Aug 22, 2006·Experimental and Molecular Pathology·Sally A DuPre', Kenneth W Hunter
Aug 25, 2007·Cancer Metastasis Reviews·Michelle D Martin, Lynn M Matrisian
Apr 2, 2008·Current Oncology Reports·Marc Beyer, Joachim L Schultze
May 27, 2008·Nature Reviews. Immunology·Weiping Zou, Lieping Chen
Sep 17, 2008·Journal of Clinical Immunology·Yisong Y Wan, Richard A Flavell
Jan 10, 2009·Nature Reviews. Cancer·Thomas Kuilman, Daniel S Peeper
Jan 24, 2009·Immunological Reviews·Craig L Maynard, Casey T Weaver
Feb 7, 2009·Nature Reviews. Immunology·Dmitry I Gabrilovich, Srinivas Nagaraj
Nov 17, 2010·Genes & Development·Thomas KuilmanDaniel S Peeper
Feb 5, 2013·Nature·Heidi BraumüllerMartin Röcken
Jun 26, 2013·Immunology and Cell Biology·Yazan S KhaledEyad Elkord

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Citations

Sep 1, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kathleen A RossBalaji Narasimhan
Nov 26, 2015·Cancer Letters·Sidra Nawaz, Yinyin Yuan
Oct 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Wei LiangNapoleone Ferrara
Jul 26, 2019·International Journal of Cancer. Journal International Du Cancer·Lothar C DieterichMichael Detmar
May 22, 2019·Frontiers in Oncology·Alyce J MayfoshMark D Hulett
Dec 7, 2019·International Journal of Cancer. Journal International Du Cancer·Hans RaskovIsmail Gögenur
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Ekaterina BayurovaMaria Isaguliants
Jul 14, 2020·Frontiers in Immunology·Sunyoung HamAndreas Möller
Mar 1, 2019·Food & Nutrition Research·Nurul Elyani MohamadNoorjahan Banu Alitheen
Feb 12, 2017·Cancer Immunology, Immunotherapy : CII·Genevieve HartleySteven Dow
Jul 24, 2018·The Tohoku Journal of Experimental Medicine·Van Minh HoNobuaki Shime
Oct 1, 2019·Cellular and Molecular Bioengineering·Samantha C SchwagerCynthia A Reinhart-King
Dec 7, 2019·Journal of Extracellular Vesicles·Shin La ShuMarc S Ernstoff
Apr 4, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Dhrubajyoti NagSubhrajit Saha
Jul 28, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masato KobayashiKiyoshi Ariizumi
Nov 17, 2020·Frontiers in Oncology·Sergi BenaventeJuan Lorente
Mar 24, 2021·Nature Immunology·Carolyn MararDenis Wirtz
Nov 18, 2021·ACS Applied Materials & Interfaces·Syed Baseeruddin AlviAravind Kumar Rengan

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Methods Mentioned

BETA
PCR
ELISA
Assay
flow cytometry

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