PMID: 7525497Dec 1, 1994Paper

Tumor cells suppress cytokine-induced nitric-oxide (NO) production in cerebral endothelial cells

International Journal of Cancer. Journal International Du Cancer
J MurataL Juillerat-Jeanneret

Abstract

Nitric oxide (NO) produced by endothelial cells (EC) has been shown to exert cytotoxic activity on tumor cells. In order to analyze events involved in brain metastasis, the modulation of NO production in rat-brain-derived EC was investigated. NO release was increased by tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-1 beta, lipopolysaccharide or forskolin in EC219 cells, a rat-brain-microvessel-derived EC line. Dexamethasone decreased NO release by cytokine-activated EC219 cells. Tumor cells (DHD/K12/PROb, a rat colon-carcinoma cell line) were highly adherent to EC219 cells, and adhesion was not modified by TNF-alpha plus IFN-gamma, or by dexamethasone. Addition of tumor cells or tumor-cell-conditioned medium significantly inhibited NO release induced by any of the stimuli examined, but only if added during the initial phase of endothelial-cell activation. Tumor-derived suppression of NO release was also observed in primary cultures of cerebral EC. NO synthase (NOS) activity in cytosol extracts of the cerebral EC line was Ca(2+)-independent and required both NADPH and tetrahydrobiopterin. NOS activity was increased by TNF-alpha and IFN-gamma, and significantly reduced by tumor-cell-conditione...Continue Reading

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Citations

Feb 24, 2000·The Journal of Pathology·F W OrrD M Nance
May 15, 2009·European Radiology·Caroline WyssXavier Montet
Jul 4, 2012·Clinical & Experimental Metastasis·Anna Sophie BerghoffRomana Höftberger
Jun 25, 1999·Free Radical Biology & Medicine·M TakenagaY Mizushima
Jan 4, 2012·Acta Neuropathologica·Matthias PreusserAndreas von Deimling
Sep 15, 1995·International Journal of Cancer. Journal International Du Cancer·J MurataL Juillerat-Jeanneret
Nov 13, 2012·Biochimica Et Biophysica Acta·Emmanouil FokasClaus Rödel
Apr 13, 2006·The Journal of Pharmacology and Experimental Therapeutics·Feride CengelliLucienne Juillerat-Jeanneret
Feb 11, 1998·Physiological Reviews·F M Faraci, D D Heistad
Feb 28, 2013·International Journal of Molecular Sciences·Yi-Wen ZangZong-You Chen

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