Tumor Detection at 3 Tesla with an Activatable Cell Penetrating Peptide Dendrimer (ACPPD-Gd), a T1 Magnetic Resonance (MR) Molecular Imaging Agent

PloS One
Christopher D MaloneQuyen T Nguyen

Abstract

The ability to detect small malignant lesions with magnetic resonance imaging (MRI) is limited by inadequate accumulations of Gd with standard chelate agents. To date, no T1-targeted agents have proven superiority to Gd chelates in their ability to detect small tumors at clinically relevant field strengths. Activatable cell-penetrating peptides and their Gd-loaded dendrimeric form (ACPPD-Gd) have been shown to selectively accumulate in tumors. In this study we compared the performance of ACPPD-Gd vs. untargeted Gd chelates to detect small tumors in rodent models using a clinical 3T-MR system. This study was approved by the Institutional-Animal Care-and-Use Committee. 2 of 4 inguinal breast fat pads of 16 albino-C57BL/6 mice were inoculated with tumor Py8119 cells and the other 2 with saline at random. MRI at 3T was performed at 4, 9, and 14 days after inoculation on 8 mice 24-hours after injection of 0.036mmol Gd/kg (ACPPD-Gd), and before and 2-3 minutes after 0.1 mmol/kg gadobutrol on the other 8 mice. T1-weighted (T1w) tumor signal normalized to muscle, was compared among the non-contrast, gadobutrol, and ACPPD-Gd groups using ANOVA. Experienced and trainee readers blinded to experimental conditions assessed for the presence ...Continue Reading

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Citations

Sep 13, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Michael T McMahon, Jeff W M Bulte
Jul 4, 2018·Contrast Media & Molecular Imaging·Min Wu, Jian Shu
May 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jing ZhaoGenxi Li
Feb 23, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Siegmund Reissmann, Margarita P Filatova
Oct 11, 2017·ACS Applied Materials & Interfaces·Jacques LuxRobert F Mattrey
Aug 16, 2021·Stem Cell Research & Therapy·Xiaoshuang ZhangHongwu Du

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Methods Mentioned

BETA
fluorescence imaging
membrane filtration
transgenic

Software Mentioned

MATLAB
R
pROC
JROC
OsiriX

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