Tumor-infiltrating effector cells of alpha-galactosylceramide-induced antitumor immunity in metastatic liver tumor

Journal of Immune Based Therapies and Vaccines
Takuya OsadaYoichi Shibata

Abstract

BACKGROUND: alpha-Galactosylceramide (alpha-GalCer) can be presented by CD1d molecules of antigen-presenting cells, and is known to induce a potent NKT cell-dependent cytotoxic response against tumor cells. However, the main effector cells in alpha-GalCer-induced antitumor immunity are still controversial. METHODS: In order to elucidate the cell phenotype that plays the most important role in alpha-GalCer-induced antitumor immunity, we purified and analyzed tumor-infiltrating leukocytes (TILs) from liver metastatic nodules of a colon cancer cell line (Colon26), comparing alpha-GalCer- and control vehicle-treated mice. Flow cytometry was performed to analyze cell phenotype in TILs and IFN-gamma ELISA was performed to detect antigen-specific immune response. RESULTS: Flow cytometry analysis showed a significantly higher infiltration of NK cells (DX5+, T cell receptor alphabeta (TCR)-) into tumors in alpha-GalCer-treated mice compared to vehicle-treated mice. The DX5+TCR+ cell population was not significantly different between these two groups, indicating that these cells were not the main effector cells. Interestingly, the CD8+ T cell population was increased in TILs of alpha-GalCer-treated mice, and the activation level of these...Continue Reading

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Citations

Jun 8, 2011·International Journal of Colorectal Disease·Matthias HornungEdward K Geissler
May 15, 2008·Expert Opinion on Biological Therapy·Elena AmbrosinoMasaki Terabe
May 14, 2011·Expert Opinion on Therapeutic Targets·Caroline C Duwaerts, Stephen H Gregory
Sep 13, 2005·Journal of Theoretical Biology·L G de PillisA E Radunskaya
Sep 24, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel G PellicciDale I Godfrey

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Methods Mentioned

BETA
flow cytometry
ELISA

Software Mentioned

CellQuest

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