PMID: 2105845Jan 1, 1990Paper

Tumor-infiltrating lymphocytes from nonrenal urological malignancies

Cancer Immunology, Immunotherapy : CII
G P HaasS A Rosenberg

Abstract

Tumor-infiltrating lymphocytes (TIL) were isolated from 15 of 20 surgical specimens of transitional cell carcinoma of the urinary bladder, prostate cancer, testicular cancer, Wilms tumor and adrenal cancer. Expansion was carried out in four different culture conditions, each containing 1000 U/ml interleukin-2: RPMI medium with or without 20% (by volume) of lymphokine-activated killer cell (LAK) supernatant and AIM V medium with or without 20% LAK supernatant. The resultant cell populations were then assayed for cytotoxicity against a variety of autologous and allogeneic tumor targets and phenotypic analysis was performed with fluorescein-labeled monoclonal antibodies. TIL growth was unrelated to the initial percentage of lymphocytes or tumor cells present in the enzymatically dispersed specimens or whether fresh or cryopreserved tissue was utilized. Better growth was seen in AIM V than in RPMI medium (P = 0.013); the beneficial effect of the addition of LAK supernatant to RPMI was indicated (P = 0.065), and the addition of LAK supernatant to AIM V did not improve the ability to culture TIL (P = 0.5) from these cancers. TIL in long-term culture were predominantly CD3+. The ratio of CD4+/CD8+ cells varied with time in culture and...Continue Reading

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Citations

Feb 27, 2001·Journal of Immunological Methods·P K WallaceM W Fanger
Mar 4, 1992·Journal of Immunological Methods·E P KaldjianK B Cease
Nov 1, 1996·Seminars in Urologic Oncology·B J GitlitzR A Figlin
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Jun 25, 2002·Expert Opinion on Biological Therapy·Bela Bodey
Dec 18, 2013·Immunological Reviews·Christian S Hinrichs, Steven A Rosenberg
Jul 1, 1992·Experimental Cell Research·D JohnstonJ C Bystryn
Feb 8, 1996·International Journal of Cancer. Journal International Du Cancer·J R YannelliS A Rosenberg
Jul 9, 2004·World Journal of Gastroenterology : WJG·Bao-Gang PengMing-De Lu
May 1, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vid LekoYong-Chen Lu
Nov 17, 2019·Journal of Surgical Oncology·Apostolos GaitanidisDhaval Patel
May 16, 1997·International Journal of Cancer. Journal International Du Cancer·F HousseauD Bellet
Sep 20, 2018·Oncoimmunology·Michael PochShari Pilon-Thomas
Sep 24, 2021·Molecular Therapy Oncolytics·Francesca PalmisaniMichael Bergmann

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