Abstract
Angiogenesis is necessary for cancer progression, but antiangiogenic therapy actually promotes tumor recurrence, progression, and metastasis. This study focused on the contribution of the tumor interstitial fluid (TIF) to lung cancer progression. TIF was isolated and quantified for 10 μg protein/mL. Malignant driver characteristics of TIF were examined by tumor-initiating cells (TIC), self-renewal, epithelial-mesenchymal transition (EMT), autophagy, and apoptosis in vitro. In vivo tumor model was used to investigate the mechanistic roles of TIF in lung cancer progression. In vitro, TIF exhibited distinct malignant driver characteristics, which led to increased numbers of TICs, increased self-renewal and EMT, as well as to decreased autophagy and apoptosis under cell starvation conditions. In vivo, the contribution of TIF was similar, as judged by increased TICs indicated by the cancer stem cell marker Nanog, the proliferation marker proliferating cell nuclear antigen, and the EMT marker N-cadherin; TIF also increased the formation of pulmonary tumors. Interestingly, the blockers of inflammation, Na-K-ATPase, and aldosterone receptor decreased TIF-induced tumor progression but increased angiogenesis. Further, we found that the w...Continue Reading
References
Jul 31, 1999·Fertility and Sterility·J A KeenanA Bukovsky
Aug 2, 2005·The Journal of Pharmacology and Experimental Therapeutics·Pierre-Luc TremblayFrançois A Auger
Jun 11, 2008·British Journal of Cancer·S M WigfieldA L Harris
Oct 22, 2009·British Journal of Cancer·K LundgrenG Landberg
Feb 18, 2010·Life Sciences·Xi-wei DingChun-ping Jiang
Apr 13, 2010·Angiogenesis·Jacobus F A JansenAmita Shukla-Dave
Jun 24, 2010·Cancer Research·Dalit BarkanJeffrey E Green
Sep 25, 2010·Cell Cycle·Barbara ChiavarinaMichael P Lisanti
Nov 20, 2010·The American Journal of Pathology·Gemma LlaveriasPhilippe G Frank
Dec 17, 2010·Cancer Research·Shih-Hwa ChiouCheng-Wen Wu
Feb 9, 2011·Physical Biology·Adrian C Shieh, Melody A Swartz
Feb 25, 2011·Genome Medicine·Bo Wang, Samson T Jacob
Apr 13, 2011·BMC Cancer·Clemens SeidelWolfgang Wick
Jun 2, 2011·Nature Reviews. Clinical Oncology·Katrien De BockPeter Carmeliet
Feb 24, 2012·Journal of Experimental & Clinical Cancer Research : CR·Yuan XuGao-Lin Liu
Jun 15, 2012·Thrombosis Research·Francesca Elice, Francesco Rodeghiero
Dec 12, 2012·Journal of Theoretical Biology·Min WuJohn Lowengrub
Aug 3, 2013·Translational Oncology·Matthias HofmannStefan Kippenberger
Aug 7, 2013·World Journal of Surgical Oncology·Shaomin LiShaoli Cheng
Aug 14, 2013·PloS One·Michael Welter, Heiko Rieger
Feb 5, 2014·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Marietta TanPatrick K Ha
Jun 3, 2014·Cancer Cell International·Gvantsa KharaishviliJan Bouchal
Jun 30, 2014·Advances in Cancer Research·Daohong ZhouDouglas R Spitz
Jul 6, 2014·Oncotarget·Elisabet CuyàsJavier A Menendez
Aug 30, 2014·Cancer Management and Research·Jennifer M Munson, Adrian C Shieh
Feb 6, 2015·OncoTargets and Therapy·Francesco SpagnoloPaola Queirolo
Apr 18, 2015·Journal of Translational Medicine·Jian WangHongzhi Sun
Oct 1, 2012·Journal of Cancer Therapy·Su HuangAhmad R Safa
May 3, 2015·Biomaterials·Peter DelNeroClaudia Fischbach
May 16, 2015·Cancer Research·Santosh Kumar GuruShashi Bhushan
May 23, 2015·Cell·Anoek ZomerJacco van Rheenen
Jun 21, 2015·Microvascular Research·M Mohammadi, P Chen