Tumor necrosis factor-alpha administration increases Kupffer cell cyclic adenosine monophosphate levels

Shock
P WangI H Chaudry

Abstract

Although studies have indicated that both Kupffer cell cyclic adenosine monophosphate (cAMP) and circulating TNF levels increase following trauma-hemorrhage and resuscitation, it remains unknown whether the elevated TNF levels are responsible for the increased Kupffer cell cAMP levels. To determine this, recombinant murine TNF-alpha (1.2 x 10(7) U/mg) was infused intravenously (.25 mg/kg body wt) over 30 min in normal rats. At 1 h after TNF-alpha or vehicle infusion, Kupffer cells and hepatocytes were isolated and cAMP levels were determined by radioimmunoassay. The levels of cAMP in the spleen and kidney were also measured. In addition, the maximal binding capacity and affinity of beta-adrenergic receptors were determined in Kupffer cells and hepatocytes by using [125I]iodopindolol. To determine whether there is any correlation between Kupffer cell cAMP and prostaglandin E2 (PGE2) or epinephrine, plasma levels of catecholamines and PGE2 were measured. The results indicated that TNF-alpha infusion significantly increased Kupffer cell cAMP levels while hepatocyte cAMP levels were not altered. Moreover, cAMP levels also increased in the macrophage/lymphocyte-rich spleen but were not altered in the kidney. Kupffer cell beta-recept...Continue Reading

Citations

Mar 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yoshio YoneyamaTsutomu Araki
Jan 18, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yoshio YoneyamaTsutomu Araki
Apr 18, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yasuko Kiyokawa, Yoshio Yoneyama
Apr 15, 2004·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Mustafa Efkan ColpanNihat Egemen
Dec 5, 2002·Shock·El Rasheid ZakariaR Neal Garrison
Dec 18, 2019·Veterinary Pathology·Ronja V KauerAnna Oevermann
Mar 14, 2000·Veterinary Pathology·P J DickinsonW Vernau

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