Tumor necrosis factor alpha and ceramide depolarise the resting membrane potential of thyroid FRTL-5 cells via a protein kinase Czeta-dependent regulation of K+ channels

Cellular Signalling
Cia RamströmK Törnquist

Abstract

Tumor necrosis factor alpha (TNFalpha) alters the electrophysiological properties of many cell types. In thyroid cells however, the effects have not yet been elucidated. Here, we report the effect of TNFalpha and its second messenger ceramide on the resting membrane potential (RMP) of thyroid FRTL-5 cells. In patch-clamp experiments, we showed that TNFalpha and ceramide depolarise the RMP by inhibiting an acid-sensitive inwardly rectifying potassium current. This depolarisation depended on the activation of protein kinase Czeta (PKCzeta), because it can be blocked by calphostin C, a PKC-inhibitory peptide and a specific inhibitor peptide for PKCzeta. The activation of PKCzeta was confirmed by Western blotting, in which a stimulation with TNFalpha led to the translocation of PKCzeta to the particulate fraction. We conclude that TNFalpha and ceramide depolarise the RMP of thyroid FRTL-5 cells by attenuating a Ba(2+)- and acid-sensitive potassium conductance via activation of PKCzeta.

References

Dec 1, 1990·The Journal of Clinical Endocrinology and Metabolism·T van der PollH P Sauerwein
Sep 1, 1989·Endocrinology·M Zakarija, J M McKenzie
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·F S Ambesi-ImpiombatoH G Coon
Jan 1, 1980·Biochimica Et Biophysica Acta·T J RinkR Y Tsien
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Sep 1, 1982·Journal of Cellular Physiology·C N Sinback, H G Coon
Oct 1, 1994·The Journal of Experimental Medicine·M G CifoneR Testi
May 1, 1994·European Journal of Endocrinology·B OngphiphadhanakulL E Braverman
Jan 1, 1997·Annual Review of Physiology·C G Nichols, A N Lopatin
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·E GulbinsF Lang
Nov 2, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M R AbrahamA Terzic
Apr 28, 2000·The Journal of Biological Chemistry·H H NietschS D Lidofsky
Mar 21, 2001·Nature Reviews. Neuroscience·S A GoldsteinN Zilberberg
Oct 26, 2001·Journal of Neurochemistry·C L ChikA K Ho

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Citations

Jun 3, 2009·Apoptosis : an International Journal on Programmed Cell Death·Ying ZhangFlorian Lang
Jun 4, 2013·Antioxidants & Redox Signaling·Laura MorenoAngel Cogolludo
Jun 4, 2011·Expert Opinion on Therapeutic Targets·Florian LangErich Gulbins
Dec 17, 2009·FEBS Letters·Elena Posse de Chaves, Simonetta Sipione
Jul 20, 2007·American Journal of Physiology. Renal Physiology·Hui-Fang BaoHe-Ping Ma
Apr 15, 2011·American Journal of Physiology. Cell Physiology·Javier Moral-SanzAngel Cogolludo
Dec 9, 2008·American Journal of Physiology. Lung Cellular and Molecular Physiology·Olivier BardouEmmanuelle Brochiero

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