Tumor necrosis factor-alpha and expression of the multidrug resistance-associated genes LRP and MRP

Journal of the National Cancer Institute
U SteinR H Shoemaker

Abstract

Cancer cells that express P-glycoprotein, multidrug resistance-associated protein (MRP), or lung resistance protein (LRP) have demonstrated resistance to a wide variety of chemotherapeutic drugs. Recently, we reported that human colon carcinoma cells that express all three proteins exhibit reduced P-glycoprotein gene expression and a loss of multidrug resistance after exposure to tumor necrosis factor-alpha, a hormone-like protein produced by cells of the immune system. In this study, we examined the effects of tumor necrosis factor-alpha on MRP and LRP gene expression in the same colon carcinoma cells. HCT15 and HCT116 colon carcinoma cells were incubated with tumor necrosis factor-alpha at 100 U/mL for 2, 12, 24, 48, or 72 hours; alternatively, cells transfected with an expression vector containing a human tumor necrosis factor-alpha complementary DNA were studied. The effects of tumor necrosis factor-alpha on MRP and LRP messenger RNA expression were evaluated by means of reverse transcription and the polymerase chain reaction; effects on MRP and LRP protein expression were examined by use of specific monoclonal antibodies and flow cytometry. The flow cytometry data were analyzed by use of the two-sided, nonparametric Mann-W...Continue Reading

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Citations

Nov 10, 2000·Biochemical and Biophysical Research Communications·C LangeU Stein
Apr 1, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Fatemeh MosaffaJavad Behravan
Dec 6, 2001·European Journal of Pharmacology·M NadaiT Hasegawa
Feb 15, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Haruyuki KawaiMine Harada
Nov 13, 2008·Cytotechnology·U Stein, W Walther
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Jan 7, 2004·Pancreas·Thomas SchaarschmidtRainer Wacke
Nov 16, 2001·Antimicrobial Agents and Chemotherapy·H AndoT Hasegawa
Jan 22, 2005·European Journal of Pharmacology·Mika MiyoshiTakaaki Hasegawa
Aug 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Ulrike SteinPeter Hohenberger
Apr 2, 2009·Expert Opinion on Therapeutic Targets·Sung Jin Ryu, Sang Chul Park
May 28, 2009·Cell Biochemistry and Function·Hong-Joo AnSang-Chul Park
Jun 22, 1999·British Journal of Cancer·T YanagisawaK Pritchard-Jones
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Dec 30, 2009·The Journal of Experimental Biology·W W DowdD Kültz
Aug 11, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Johan RenesMichael Müller

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