PMID: 2115318Jan 1, 1990Paper

Tumor necrosis factor-alpha inhibits cell proliferation and induces class II antigens and cell adhesion molecules in cultured normal human keratinocytes in vitro

Archives of Dermatological Research
M Detmar, C E Orfanos

Abstract

The effects of recombinant human tumor necrosis factor-alpha (TNF) on cell proliferation, cell morphology, and on the expression of class II alloantigens and intercellular adhesion molecule-1 (ICAM-1) were assessed in human keratinocytes cultured in serum-free medium. TNF inhibited cell proliferation in a time- and dose-dependent manner with a minimum effective dose of 10 U/ml and a 50% inhibitory dose of 100 U/ml. However, TNF did not induce cell death, and the growth inhibition induced by TNF was completely reversible after its withdrawal. In vitro combination of TNF with interferon-alpha (IFN-alpha) and IFN-beta resulted in additive growth inhibitory effects, while IFN-gamma enhanced the TNF mediated growth inhibition in a synergistic way. Furthermore, TNF altered the morphology of the growing keratinocytes inducing the appearance of a fusiform, fibroblast-like population. Also, treatment with TNF over 6 days markedly induced the expression of ICAM-1 on the cultured keratinocytes with a minimal effective dose of 10 U/ml, while HLA-DR was only moderately expressed after 1,000 U/ml. TNF did not induce HLA-DQ, but reduced the IFN-gamma induced expression of HLA-DR and HLA-DQ. By immunoelectron microscopy, an intense membrane-bo...Continue Reading

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Citations

Aug 25, 2011·Archives of Dermatological Research·Cheryl M SweeneyBrian Kirby
Sep 8, 1998·Journal of Reproductive Immunology·G Ljunggren, D J Anderson
Jan 13, 2009·Skin Pharmacology and Physiology·S-P SongM Q Man
Dec 29, 2013·Mediators of Inflammation·Haur Yueh LeeMasato Kakeda
Jun 15, 2010·Journal of the American Academy of Dermatology·David A Weinstein, Robert S Kirsner
Dec 17, 2014·The Journal of Investigative Dermatology·Xiaoling ZhangJennifer Y Zhang
Jul 9, 2004·Photodermatology, Photoimmunology & Photomedicine·Hui DengKang-huang Liao
Aug 31, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Allison J CowinDavid A Belford
Oct 5, 2013·The American Journal of Pathology·Margaret L Novak, Timothy J Koh
Dec 12, 2017·The Journal of Dermatology·Eisaku OgawaRyuhei Okuyama
Feb 3, 2012·International Wound Journal·Julia EscandonStephen Davis
Oct 21, 2000·Endocrine Reviews·A Slominski, J Wortsman
Sep 13, 2020·International Journal of Molecular Sciences·Yuki SatoRyuhei Okuyama
Jan 1, 1992·The British Journal of Dermatology·B S Baker, L Fry
Dec 11, 2020·American Journal of Clinical Dermatology·Kevin YangBoni E Elewski
Dec 5, 2020·Scientific Reports·Jessica E NeilAdrian C Williams
Nov 1, 1993·Infection and Immunity·M W OllertW Bräutigam

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