Tumor necrosis factor alpha is necessary for granulocyte-macrophage-colony-stimulating-factor-induced eosinophil transendothelial migration

International Archives of Allergy and Immunology
R A Erger, T B Casale

Abstract

We have previously shown that granulocyte macrophage-colony stimulating factor (GM-CSF) was capable of inducing eosinophil migration across naked filters but not endothelial monolayers. Tumor necrosis factor alpha (TNF-alpha) has been shown to be a key factor in granulocyte adhesion and transendothelial migration. We, therefore, pretreated human umbilical vein endothelial cell (HUVEC) monolayers with TNF-alpha and studied whether TNF-alpha could support GM-CSF-induced eosinophil transendothelial migration. We found that TNF-alpha supported GM-CSF-induced eosinophil transendothelial migration and that this process was: (1) dependent upon GM-CSF and TNF-alpha dose; (2) time-dependent; (3) not due to TNF-alpha having a chemotactic effect itself; (4) not due to TNF-alpha-induced soluble factor production by endothelium, and (5) inhibitable by actinomycin D. We next studied the specificity of this response. Neutrophils did not migrate across TNF-alpha-pretreated endothelium in response to GM-CSF. TNF-alpha pretreatment of A549 human type-II-like epithelial lung cells (A549) did not support GM-CSF-induced transepithelial migration. Neither interleukin (IL)-1 nor GM-CSF pretreatment of the HUVEC supported GM-CSF-induced transendotheli...Continue Reading

Citations

Jan 3, 2001·Environmental Health Perspectives·W DongM I Gilmour
May 18, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E FurukawaH Sasaki
Feb 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W K IpC W K Lam
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S ShahabuddinR P Schleimer

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