Tumor necrosis factor-alpha mediates polymethylmethacrylate particle-induced NF-kappaB activation in osteoclast precursor cells

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
John C ClohisyY Abu-Amer

Abstract

Tumor necrosis factor-alpha (TNF) is a potent osteoclastogenic cytokine that has a fundamental role in the pathogenesis of implant particle-induced osteolysis. The nuclear transcription factor NF-kappaB mediates TNF signaling and this transcription complex is necessary for osteoclastogenesis. Because polymethylmethacrylate (PMMA) particles cause osteolysis, we reasoned the PMMA would induce NF-kappaB activation. In fact, we find that exposure of osteoclast precursors, in the form of colony stimulating factor-1 (CSF-1) dependent murine bone marrow macrophages, to PMMA particles prompts nuclear translocation and activation of NF-kappaB. Supershift assays confirm the presence of the p50 and p65 NF-kappaB subunits in the activated transcription factor. Particle-induced NF-kappaB activation is equal in both wild type and LPS- hyporesponsive cells indicating that the phenomenon does not represent endotoxin contamination. A soluble, competitive inhibitor of TNF (huTNFr:Fc) dampens particle-directed NF-kappaB activation and this response is also abrogated in TNF-/- osteoclast precursors. Thus, PMMA particle activation of NF-kappaB is a secondary event resulting from enhanced TNF expression and is independent of LPS contamination.

References

Feb 24, 1995·Cell·D Thanos, T Maniatis
Jun 1, 1993·The Journal of Bone and Joint Surgery. American Volume·D R HaynesD W Howie
Oct 4, 1996·Cell·P A Baeuerle, D Baltimore
Sep 18, 1997·The Journal of Clinical Investigation·Y Abu-AmerS L Teitelbaum
Nov 14, 1997·Nature Medicine·Y Abu-Amer, M M Tondravi
Nov 14, 1997·Nature Medicine·V IotsovaR Bravo
Jan 23, 1999·The American Journal of Pathology·K D MerkelS L Teitelbaum
Jun 9, 1999·The Journal of Bone and Joint Surgery. American Volume·K A RoebuckT T Glant
Jan 13, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A A RagabE M Greenfield

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Citations

Jun 20, 2003·Experimental Gerontology·Bruce R Troen
Jul 28, 2011·Cancer Biotherapy & Radiopharmaceuticals·Roger LoriaPeter Guida
Jul 20, 2007·Arthritis Research & Therapy·Yousef Abu-AmerJohn C Clohisy
Sep 21, 2012·Canadian Journal of Surgery. Journal Canadien De Chirurgie·Shahryar Noordin, Bassam Masri
Oct 21, 2005·The Keio Journal of Medicine·Brendan F BoyceLianping Xing
Apr 8, 2011·The Journal of Surgical Research·Qing FangQingsheng Zhu
Oct 12, 2004·Biomaterials·Eileen Ingham, John Fisher
Aug 8, 2006·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kenjiro KogaKazuhiro Shintani
Mar 22, 2016·Biochemical and Biophysical Research Communications·Naicheng LiuJianning Zhao
Sep 25, 2004·Journal of Cellular Biochemistry·Yousef Abu-AmerTeruhisa Hirayama
Dec 6, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John C ClohisyYousef Abu-Amer
Feb 6, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John C ClohisyYousef Abu-Amer
Oct 14, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Edward M SchwarzR John Looney
May 30, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Y YamanakaJ C Clohisy
May 18, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·John C ClohisyYousef Abu-Amer
Jul 11, 2007·Clinical Orthopaedics and Related Research·G HoltR M D Meek
Jul 26, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yasuhiro YamanakaYousef Abu-Amer
Aug 21, 2010·Journal of Biomedical Materials Research. Part a·Li MaoqiangTang Tingting
Jan 5, 2006·Journal of Biomedical Materials Research. Part a·Elizabeth A FritzKenneth A Roebuck
Dec 14, 2005·Current Opinion in Rheumatology·R John LooneyRegis J O'Keefe
May 26, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Bernd BaumannChristof P Rader
Jun 14, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Elizabeth A FritzKenneth A Roebuck
Apr 24, 2015·Proceedings. Biological Sciences·Mark Anthony BrowneJan A van Franeker
Sep 28, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Edward M GreenfieldJoscelyn M Seabold
May 14, 2011·The Journal of Biological Chemistry·Yasuhiro YamanakaYousef Abu-Amer
Aug 15, 2002·The Journal of Bone and Joint Surgery. American Volume·Matthew J Silva, Linda J Sandell
Feb 23, 2020·Frontiers in Immunology·Christian GuderFrank A Schildberg
Nov 11, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·John C ClohisyYousef Abu-Amer
Oct 14, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S AbbasY Abu-Amer

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