Tumor necrosis factor alpha: posttranscriptional stabilization of WAF1 mRNA in p53-deficient human leukemic cells

Journal of Cellular Physiology
M ShioharaH P Koeffler

Abstract

The p53 protein directly regulates the expression of the WAF1 (wild-type p53-activated fragment 1) protein which is a cyclin-dependent kinase inhibitor (CDK1). DNA damaging agents such as ionizing or UV radiation, and some chemical agents induce WAF1 in wild-type p53 containing cells, thereby halting cell cycle progression. WAF1 expression is also induced through a p53-independent pathway. Tumor necrosis factor alpha (TNF alpha) is a cytotoxic/cytostatic compound for some human cancer cells. We examined a series of myeloid leukemic cell lines that expressed either no p53 (HL-60, K562) or mutant inactive p53 (KG-1, KCL22,THP-1, U937). The KG-1, HL-60, K562, and KCL22 myeloid leukemic cells increased their levels of WAF1 mRNA in the presence of TNF alpha. We focused on KG-1 cells to determine how TNF alpha modulated WAF1 expression. WAF1 mRNA increased in a dose-dependent manner in the cells after exposure to increasing concentrations of TNF alpha, and this increase occurred in the absence of new protein synthesis. An increase of WAF1 protein and a concominant decrease of cyclin-dependent kinase 2 activity also was found in KG-1 cells. Flow cytometry using 5-bromo-2'-deoxyuridine showed an increase in the proportion of TNF alpha-...Continue Reading

References

May 15, 1976·International Journal of Cancer. Journal International Du Cancer·C Sundström, K Nilsson
Jan 1, 1990·The Journal of Clinical Investigation·M AkashiH P Koeffler
Mar 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D CaputA Cerami
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D Wolf, V Rotter
May 1, 1987·European Journal of Immunology·G B DealtryF R Balkwill
Dec 16, 1987·Biochemical and Biophysical Research Communications·P SuffysW Fiers
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P DefilippiJ Content
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Aug 1, 1980·International Journal of Cancer. Journal International Du Cancer·S TsuchiyaK Tada
Dec 1, 1995·Journal of Cellular Physiology·T NakamakiH P Koeffler
Aug 1, 1994·Genes & Development·H ZhangD Beach
Mar 1, 1994·Molecular and Cellular Biology·H MatsushimeJ Y Kato
Nov 19, 1993·Cell·W S el-DeiryB Vogelstein
Dec 3, 1993·Cell·T Hunter
Dec 16, 1993·Nature·Y XiongD Beach
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L SzekelyK G Wiman

❮ Previous
Next ❯

Citations

Nov 8, 2003·International Journal of Hematology·Hiroaki GotoShumpei Yokota
May 30, 1998·Biochemical Pharmacology·C Stecca, G B Gerber
Dec 4, 2001·Biochimica Et Biophysica Acta·S DaubeufM M Galteau
Jun 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Mei NieKarl Matter
Dec 16, 2010·The Journal of Biological Chemistry·Sunil K MannaMaikho Thoh
Mar 21, 1998·Clinical Obstetrics and Gynecology·M Akashi, H P Koeffler
Jun 1, 1997·Leukemia & Lymphoma·M ShioharaH P Koeffler
Oct 6, 2007·Expert Opinion on Biological Therapy·Don Benjamin, Christoph Moroni
Jun 11, 2002·Biochemical and Biophysical Research Communications·Masaaki ShioharaJunji Sagara
Sep 18, 1998·Biochimica Et Biophysica Acta·K FukuchiK Gomi
Nov 2, 1999·Virology·P J Duerksen-HughesS B Schwartz
May 29, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J E BelizárioW Beçak
Sep 1, 2016·PloS One·Gerd BirkenmeierFaramarz Dehghani
Jun 21, 2005·The Journal of Biological Chemistry·Hongyan ZhuCarl G Maki
Dec 22, 1999·The Journal of Biological Chemistry·J R Biggs, A S Kraft
Jun 2, 2007·Molecular Cancer Therapeutics·Takayuki IkezoeHirokuni Taguchi
Feb 13, 2007·Biochemical and Biophysical Research Communications·Yumei FanZhimin Yin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.