Tumor necrosis factor-alpha regulates the gene expression of macrophage migration inhibitory factor through tyrosine kinase-dependent pathway in 3T3-L1 adipocytes

Journal of Biochemistry
J HirokawaJ Nishihira

Abstract

Macrophage migration inhibitory factor (MIF) has been rediscovered as a proinflammatory cytokine, pituitary hormone, and glucocorticoid-induced immunoregulator. We have recently identified the expression of MIF in adipocytes and found that tumor necrosis factor (TNF)-alpha stimulates its secretion from 3T3-L1 adipocytes. Since adipocytes are regarded as a potential source of various biologically active substances, we examined in more detail the effect of TNF-alpha on MIF expression in 3T3-L1 adipocytes in the present study. We found that TNF-alpha induced MIF mRNA in dose- and time-dependent manners. After stimulation with TNF-alpha, the amount of intracellular MIF protein was unchanged or slightly decreased, concomitant with increased release of this protein into the extracellular space. This observation indicates that TNF-alpha stimulates MIF secretion from the constitutively expressed intracellular pool of 3T3-L1 adipocytes and promotes de novo synthesis of MIF. From evaluation of the mechanism of MIF gene expression, we found that tyrosine kinase inhibitors, either genistein or herbimycin A, suppressed the MIF mRNA induction by TNF-alpha. The results suggest the possibility that upregulation of MIF mRNA expression by TNF-al...Continue Reading

Citations

May 17, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Tullia TodrosLuana Paulesu
Sep 6, 2002·Pharmacology, Biochemistry, and Behavior·Marta PuertoMónica De la Fuente
Dec 22, 1999·Immunopharmacology·R CorreaM De La Fuente
May 30, 2001·Nutrition·U N Das
Aug 24, 2012·The Proceedings of the Nutrition Society·Orla M FinucaneHelen M Roche
Dec 4, 2008·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J Ye
Sep 3, 2011·Clinical and Experimental Pharmacology & Physiology·Chao-Yu Miao
Jan 27, 2007·BMC Bioinformatics·Manisha BrahmacharyVladimir B Bajic
Jan 18, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Y Suresh, Undurti N Das
Sep 26, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·J A VillenaL Casteilla
Dec 30, 2018·Scandinavian Journal of Immunology·Gholamreza DaryaborKurosh Kalantar
Jun 7, 2006·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Baha Al-ShawwaMutasim Abu-Hasan
Aug 2, 2007·American Journal of Physiology. Endocrinology and Metabolism·Jianping YeQing He
Aug 22, 2017·Frontiers in Endocrinology·Undurti N Das
Aug 16, 2008·Endocrinology·Daisuke IkedaMasaharu Nishimura
Mar 28, 2001·Annals of the New York Academy of Sciences·N Petrovsky, R Bucala
Sep 9, 2015·PloS One·Bong-Sung KimNorbert Pallua
Dec 2, 2020·Scientific Reports·Rodrigo Mohallem, Uma K Aryal
Jul 4, 2006·Nutrition·Undurti N Das
Oct 1, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T G RamsayT J Caperna

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.