Tumor necrosis factor induced release of endothelial cell lipoprotein lipase

Arteriosclerosis : an Official Journal of the American Heart Association, Inc
U SaxenaI J Goldberg

Abstract

The major functional pool of lipoprotein lipase (LPL) that hydrolyzes triglycerides in circulating lipoproteins is located on the vascular endothelium. The macrophage-secreted cytokine tumor necrosis factor (TNF), a molecule known to affect endothelial cell functions, was used to test the hypothesis that alterations of endothelial cell metabolism regulate the binding of LPL to these cells. TNF addition induced rapid (maximum release at 45 minutes) dissociation of LPL protein and activity from its binding sites on cultured porcine aortic endothelial cells. LPL release by TNF required endothelial cell metabolic event(s) which involved cell secretion. In addition, LPL release was inhibited by pertussis toxin, suggesting the involvement of guanine nucleotide regulatory protein(s). Addition of arachidonic acid, a molecule known to be released by endothelial cells due to phospholipase A2 activation by TNF treatment, released LPL from the cell surface. Furthermore, direct modulation of cellular phospholipase A2 activity also led to changes in the release of LPL. Our studies demonstrate that alterations in the cellular metabolism of endothelial cells, for example, by TNF, may release functional pools of LPL from the vascular endotheliu...Continue Reading

References

May 1, 1976·The Journal of Infectious Diseases·R L KaufmannW R Beisel
Feb 20, 1989·Biochimica Et Biophysica Acta·T Chajek-ShaulY Stein
Jun 1, 1988·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M J Mooibroek, J H Wang
Jan 1, 1986·Annual Review of Cell Biology·L Stryer, H R Bourne
Aug 26, 1988·Science·J D EskoJ L Weinke
Oct 1, 1988·The Journal of Clinical Investigation·H F StarnesM F Brennan
Mar 1, 1988·The Journal of Experimental Medicine·K J TraceyA Cerami
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Jul 1, 1987·The Journal of Experimental Medicine·K A HajjarR L Nachman
Mar 1, 1986·The Journal of Experimental Medicine·P P Nawroth, D M Stern
Jan 1, 1985·Preparative Biochemistry·L SocorroR L Jackson
Oct 9, 1970·Biochemical and Biophysical Research Communications·J C LaRosaD S Fredrickson
May 1, 1968·Journal of Bacteriology·D Farshtchi, V J Lewis
Nov 13, 1969·The New England Journal of Medicine·J I GallinW M O'Leary
Nov 29, 1984·The New England Journal of Medicine·C A Dinarello
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G L KingC H Heldin
Nov 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·J A CornicelliD S Goodman
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M KawakamiA Cerami

❮ Previous
Next ❯

Citations

Apr 13, 2000·Biochimica Et Biophysica Acta·M Szewczenko-Pawlikowski, W Kozak
Aug 19, 1991·International Journal of Cancer. Journal International Du Cancer·P MatthysA Billiau
Jul 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·J C ObunikeI J Goldberg
Sep 26, 2001·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·N G ForouhiP M McKeigue
Jul 1, 1996·British Journal of Obstetrics and Gynaecology·N SattarI A Greer
Jan 3, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Grith L SorensenUffe Holmskov
Sep 6, 2000·Clinical and Experimental Hypertension : CHE· Sheu WHHY T Chen
Jun 16, 2021·Atherosclerosis·Ira J GoldbergNada A Abumrad
Feb 1, 2005·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Eduardo EsteveJosé Manuel Fernández-Real

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.