Tumor Necrosis Factor Inhibition in the Acute Management of Traumatic Optic Neuropathy

Investigative Ophthalmology & Visual Science
Brian C TseDaniel Pelaez

Abstract

To determine the effectiveness of etanercept, a tumor necrosis factor (TNF) inhibitor, in conferring neuroprotection to retinal ganglion cells (RGCs) and improving visual outcomes after optic nerve trauma with either optic nerve crush (ONC) or sonication-induced traumatic optic neuropathy (SI-TON) in mice. Mouse optic nerves were unilaterally subjected to ONC (n = 20) or SI-TON (n = 20). TNF expression was evaluated by using immunohistochemistry and quantitative RT-PCR (qRT-PCR) in optic nerves harvested 6 and 24 hours post ONC (n = 10) and SI-TON (n = 10). Mice in each injury group received daily subcutaneous injections of either etanercept (10 mg/kg of body weight; five mice) or vehicle (five mice) for 7 days. Pattern electroretinograms were performed on all mice at 1 and 2 weeks after injury. ONC mice were killed at 2 weeks after injury, while SI-TON mice were euthanized at 4 weeks after injury. Whole retina flat-mounts were used for RGC quantification. Immunohistochemistry and qRT-PCR showed upregulation of TNF protein and gene expression within 24 hours after injury. In both models, etanercept use immediately following optic nerve injury led to higher RGC survival when compared to controls, which was comparable between the...Continue Reading

References

Sep 1, 1996·Ophthalmic Plastic and Reconstructive Surgery·R A Goldberg, K D Steinsapir
Jul 7, 1998·Toxicology and Applied Pharmacology·B VivianiM Marinovich
Jan 6, 2000·Current Opinion in Ophthalmology·K D Steinsapir
Feb 17, 2000·The Annals of Pharmacotherapy·J M Korth-BradleyM E Lebsack
Mar 16, 2000·Trends in Neurosciences·H D VentersK W Kelley
Jul 20, 2000·Journal of Neuroimmunology·M LiefnerW Brück
Jan 18, 2003·Journal of Neurology, Neurosurgery, and Psychiatry·A CartaF Carta
Mar 5, 2003·American Journal of Ophthalmology·Felipe A MedeirosRemo Susanna
Nov 10, 2004·Eye·H Levkovitch-Verbin
Dec 28, 2006·European Journal of Ophthalmology·A CartaS Gomarasca
Aug 28, 2010·Journal of Neurochemistry·Chung-Ching ChioChing-Ping Chang
May 2, 2012·Molecular and Cellular Biology·Christine RichterAnja Krippner-Heidenreich
May 17, 2012·Investigative Ophthalmology & Visual Science·Giedrius KalesnykasHarry A Quigley
Jan 22, 2013·Expert Opinion on Drug Safety·Giuseppe MurdacaFrancesco Puppo
Jun 20, 2013·The Cochrane Database of Systematic Reviews·Patrick Yu-Wai-Man, Philip G Griffiths
Mar 22, 2014·The Journal of Infectious Diseases·Jing YeShengbo Cao
Mar 29, 2014·Investigative Ophthalmology & Visual Science·Tsung-Han ChouVittorio Porciatti
Apr 20, 2014·JAMA Ophthalmology·William Marshall GuyRobert B Parke
Apr 29, 2014·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Murat TopdagPervin Iseri
Sep 19, 2014·Neurosurgery·Christopher C Giza, David A Hovda
Feb 25, 2015·Craniomaxillofacial Trauma & Reconstruction·Arjunan Muthu KumaranLim Thiam Chye
Aug 27, 2015·Journal of the Peripheral Nervous System : JPNS·Pinelopi TsouniThierry Kuntzer
Oct 9, 2015·Current Opinion in Ophthalmology·Benjamin C Chaon, Michael S Lee
Dec 15, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Pernille M MadsenKate L Lambertsen
Jan 14, 2016·Military Medical Research·Eric L SingmanTimothy McCulley
Jun 9, 2016·The British Journal of Ophthalmology·Wentao YanWencan Wu
Aug 27, 2016·The European Journal of Neuroscience·Galina DvoriantchikovaDmitry Ivanov
Jun 28, 2017·Journal of Clinical & Cellular Immunology·Ashley E RussellJames W Simpkins
Oct 6, 2017·Investigative Ophthalmology & Visual Science·Yasushi KitaokaHitoshi Takagi
Oct 8, 2017·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Mohsen Bahmani KashkouliMansoureh Bagheri

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

TON
SI

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.