Tumor necrosis factor receptor-1 is essential for LPS-induced sensitization and tolerance to oxygen-glucose deprivation in murine neonatal organotypic hippocampal slices

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
Tina MarkusDavid Ley

Abstract

Inflammation and ischemia have a synergistic damaging effect in the immature brain. The role of tumor necrosis factor (TNF) receptors 1 and 2 in lipopolysaccharide (LPS)-induced sensitization and tolerance to oxygen-glucose deprivation (OGD) was evaluated in neonatal murine hippocampal organotypic slices. Hippocampal slices from balb/c, C57BL/6 TNFR1(-/-), TNFR2(-/-), and wild-type (WT) mice obtained at P6 were grown in vitro for 9 days. Preexposure to LPS immediately before OGD increased propidium iodide-determined cell death in regions CA1, CA3, and dentate gyrus from 4 up to 48 h after OGD (P<0.001). Extending the time interval between LPS exposure and OGD to 72 h resulted in tolerance, that is reduced neuronal cell death after OGD (P<0.05). Slices from TNFR1(-/-) mice showed neither LPS-induced sensitization nor LPS-induced tolerance to OGD, whereas both effects were present in slices from TNFR2(-/-) and WT mice. Cytokine secretion (TNFalpha and interleukin-6) during LPS exposure was decreased in TNFR1(-/-) slices and increased in TNFR2(-/-) as compared with WT slices. We conclude that LPS induces sensitization or tolerance to OGD depending on the time interval between exposure to LPS and OGD in murine hippocampal slice cul...Continue Reading

References

Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Jan 15, 1993·Brain Research. Developmental Brain Research·D MullerL Stoppini
May 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H NawashiroJ M Hallenbeck
Jul 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J IvackoF S Silverstein
Mar 28, 1998·European Journal of Radiology·P Anslow
Apr 14, 2000·Current Opinion in Pediatrics·O Dammann, A Leviton
Aug 19, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R BordetB Dupuis
Nov 9, 2000·The European Journal of Neuroscience·G J WildeF Iannotti
Apr 4, 2001·The European Journal of Neuroscience·S EklindH Hagberg
Mar 14, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Paula M HughesChristoph Wiessner
Mar 1, 2003·Neurochemical Research·Carina MallardIngemar Kjellmer
May 9, 2003·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·D OgunyemiB Alperson
Feb 14, 2004·Stroke; a Journal of Cerebral Circulation·Tobias CronbergTadeusz Wieloch
Nov 17, 2004·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Katalin KarikóFrank A Welsh
Mar 25, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David StellwagenRobert C Malenka
Apr 19, 2005·Journal of Neuroimmunology·Reiko KunoAkio Suzumura
Jun 1, 2005·Journal of Neuroscience Methods·David Fernández-LópezIgnacio Lizasoain
Jul 22, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Liliana BernardinoJens Zimmer
Sep 27, 2005·Pediatric Research·Ingrid Hansen-PuppDavid Ley
Dec 14, 2005·Neuroscience and Behavioral Physiology·T D VlasovE A Polyakova
Jun 24, 2006·Seminars in Fetal & Neonatal Medicine·Xiaoyang WangCarina Mallard
Mar 1, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Holly L RosenzweigMary P Stenzel-Poore

❮ Previous
Next ❯

Citations

Oct 26, 2012·ISRN Neurology·Carina Mallard
Mar 1, 2012·Neurology Research International·Claire ThorntonHenrik Hagberg
Feb 11, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Claire Thornton, Henrik Hagberg
Mar 25, 2015·Developmental Medicine and Child Neurology·Bobbi FleissPierre Gressens
Aug 21, 2012·American Journal of Obstetrics and Gynecology·Alan Leviton
Dec 8, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Giles S KendallGennadij Raivich
Sep 8, 2017·Frontiers in Cellular Neuroscience·Claire ThorntonHenrik Hagberg

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
light microscopy
flow cytometry

Software Mentioned

SPSS
Image
Pro Plus
BD Biosciences CBA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
U ScherbelT K McIntosh
Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology
Krishnan Sriram, James P O'Callaghan
© 2022 Meta ULC. All rights reserved