PMID: 6974741Oct 1, 1981Paper

Tumor promoter phorbol myristic acetate stimulates immunoglobulin secretion correlated with growth cessation in human B lymphocyte cell lines

The Journal of Clinical Investigation
P Ralph, T Kishimoto

Abstract

Immunoglobulin production by lymphoblast cell lines was studies using protein A-red blood cell plaque formation to detect individual secreting cells. Immunoglobulin (Ig) secretion by 6 of 12 human B-cell lines tested could be stimulated up to twentyfold by phorbol myristic acetate (PMA) at subtoxic concentrations of 10-1000 ng/ml depending on the line. Stimulation was found with both IgM and IgG cell lines. No switch of Ig class synthesis was found in the cell lines as a result of PMA incubation. Increase in Ig secretion was closely associated with cessation of growth resembling induction of terminal differentiation in the cells. PMA induction of Ig secretion in B lymphocytes from normal peripheral blood requires the cooperation of T cells. PMA stimulation of certain cell lines reported here suggests that the lines are late in the differentiation pathway to plasmacyte and can be easily triggered to secrete Ig by membrane-altering agents.

References

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Citations

Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R A FeldmanH Hanafusa
Dec 1, 1985·Scandinavian Journal of Immunology·M J Dauphinée, N Talal
Sep 1, 1989·Journal of Cellular Physiology·M R Pittelkow, G D Shipley

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