PMID: 6171814Oct 1, 1981Paper

Tumor promoters alter gene expression and protein phosphorylation in avian cells in culture

Proceedings of the National Academy of Sciences of the United States of America
A LaszloM J Bissell

Abstract

We have investigated the effect of 12-O-tetradecanoylphorbol 13-acetate (TPA) on the synthesis and modification of polypeptides in normal avian cells and cells infected by wild-type and temperature-sensitive Rous sarcoma virus (RSV). Using two-dimensional gel electrophoresis, we have detected alterations in both the abundance of cellular polypeptides and in their phosphorylation that seem unique to TPA treatment. However, the state of phosphorylation of the major putative substrate for the action of the src gene-associated protein kinase, the 34- to 36-kilodalton protein, was not altered. Moreover, examination of the phosphorylated amino acid content of total cellular phosphoproteins revealed that the response to TPA was not associated with detectable increases in their phosphotyrosine content. These results make it unlikely that TPA acts by the activation of the phosphorylating activity of the cellular proto-src gene or by the activation of other cellular phosphotyrosine-specific kinases. We have shown previously that temperature-sensitive RSV-infected cells at nonpermissive temperature demonstrate an increased sensitivity to TPA treatment [Bissell, M. J., Hatie, C. & Calvin, M. (1979) Proc. Natl. Acad. Sci. USA 76, 348-352]. ...Continue Reading

References

Jan 1, 1977·Journal of Supramolecular Structure·M J BissellA S Tung
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M S Collett, R L Erikson
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M J BissellM Calvin
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H OppermannJ M Bishop
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M S CollettR L Erikson
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A F LauA J Faras
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K Radke, G S Martin
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Hunter, B M Sefton
Nov 1, 1980·The Journal of Cell Biology·R L EriksonJ S Brugge
Jul 20, 1964·Archives of Biochemistry and Biophysics·H K MITCHELL, K D LUNAN

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Citations

Jun 1, 1992·Journal of Cellular Physiology·A LaszloJ L Roti Roti
Jan 1, 1984·Journal of Cancer Research and Clinical Oncology·H Busch
Sep 1, 1987·Collagen and Related Research·C C ChuaR L Ladda
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S O MoonA C King
Feb 15, 1983·European Journal of Biochemistry·A WeberA Kahn
Jun 14, 1985·Biochemical and Biophysical Research Communications·J Viviers, J C Schabort
Oct 1, 1983·Experimental Cell Research·A Laszlo, M J Bissell
Sep 15, 1983·Biochemical and Biophysical Research Communications·R BishopM J Weber
Apr 1, 1987·Journal of Cellular Biochemistry·A H LockwoodM Pendergast
Jul 15, 1999·Cancer Detection and Prevention·M KouloukoussaE Marinos
Sep 1, 1985·Molecular and Cellular Biology·R BishopH R Herschman

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