Tumor-promoting phorbol ester induces alterations of sialidase and sialyltransferase activities of JB6 cells

Japanese Journal of Cancer Research : Gann
Taeko MiyagiS Tsuiki

Abstract

Sialidase and sialyltransferase activities were studied in JB6 mouse epidermal cells before and after exposure to phorbol ester, 12-O-tetradecanoyl phorbol-13-acetate (TPA), which irreversibly induces anchorage-independent growth and tumorigenicity. JB6 cells exhibited sialidase activities toward 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid (4MU-NeuAc) and gangliosides at pH 4.5 in the particulate fraction but apparently not in the cytosol at pH 4.5 or 6.0. In JB6 cells exposed to TPA and in the anchorage-independent transformants, the sialidase activity toward 4MU-NeuAc was decreased and the activity toward gangliosides was increased compared with those in untreated JB6 cells. Immunological analysis with antisera against membrane-associated sialidases I and II revealed that plasma membrane-associated sialidase I was increased and lysosomal membrane-associated sialidase II was decreased under these conditions. TPA treatment also affected the sialyltransferase activities of JB6 cells: and elevation of the transfer activities toward asialo-orosomucoid and asialo-porcine submaxillary mucin but a reduction of GM3 and GD3 synthase activities were observed on exposure to TPA and in cells transformed by TPA to retain anchorage...Continue Reading

References

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Citations

Apr 22, 1999·Cancer Letters·H SönmezE Kökoglu
Jul 18, 2008·Journal of Biochemistry·Taeko MiyagiKazuhiro Shiozaki
Dec 17, 2008·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Taeko Miyagi
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoichiro KakugawaTaeko Miyagi
Nov 21, 2007·Biochimica Et Biophysica Acta·Taeko MiyagiKazunori Yamaguchi
Jul 25, 2008·Proteomics·Taeko MiyagiTsuneaki Fujiya
Nov 19, 2002·The Tohoku Journal of Experimental Medicine·Naile OzenEmine Kokoglu
Apr 29, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jishun Lu, Jianguo Gu
May 16, 2002·The Journal of Biological Chemistry·Yan WangTaeko Miyagi
Jul 16, 1999·Biochemical and Biophysical Research Communications·T WadaT Miyagi

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