Tumor-promoting properties of miR-8084 in breast cancer through enhancing proliferation, suppressing apoptosis and inducing epithelial-mesenchymal transition

Journal of Translational Medicine
Yujing GaoPu Li

Abstract

Breast cancer is one of the most frequent malignancies and the second leading cause of cancer-related mortality in women. MicroRNAs play a key role in breast cancer development and progression. microRNA(miR)-8084 has been observed an aberrant expression in breast cancer. However, the functions and regulatory axes of miR-8084, particularly in breast cancer, were not entirely clear. miR-8084 expression in breast cancer were investigated in a GEO dataset by in silico analysis and in 42 paired tumor tissues by qPCR. The effects of deregulation of miR-8084 on breast cancer cell proliferation, migration and invasion in vitro and tumorigenicity in vivo were examined by colony-formation assay, wound healing assay, transwell assay and nude mouse subcutaneous tumor formation model. The target gene of miR-8084 were predicted by TargetScan and miRDB, and confirmed by luciferase reporter system. The roles of miR-8084 in the breast cancer cell proliferation, apoptosis and epithelial-mesenchymal transition (EMT) were investigated by MTS, FACS and associated-marker detection by western blot. miR-8084 is significantly up-regulated in both serum and malignant tissues from the source of breast cancer patients. miR-8084 promotes the proliferation ...Continue Reading

References

Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·M NagashimaC C Harris
Sep 10, 2004·Molecular Biology and Evolution·Gordon H Y HeKarl Riabowol
Jul 19, 2005·Molecular and Cellular Biology·Remy PedeuxCurtis C Harris
Aug 27, 2005·Oncogene·Jiri Zavadil, Erwin P Böttinger
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
May 27, 2006·Nature·Pedro V PeñaTatiana G Kutateladze
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Mar 13, 2008·The Journal of Biological Chemistry·Krishna P SarkerShirin Bonni
Dec 4, 2008·Gastroenterology·Steven P O'HaraNicholas F LaRusso
Oct 6, 2009·The International Journal of Biochemistry & Cell Biology·Kimberly Breving, Aurora Esquela-Kerscher
Jul 29, 2010·Cancer Research·Derek M Dykxhoorn
Nov 10, 2010·Clinical Chemistry·Claire CorcoranLorraine O'Driscoll
Dec 3, 2010·The Journal of Pathology·Alessandro Bombonati, Dennis C Sgroi
Dec 4, 2012·International Journal of Molecular Sciences·Joy TangFazlul H Sarkar
Jun 11, 2014·International Journal of Oncology·Kaiyuan ZhangJiqiang Zhang
Nov 8, 2014·Nucleic Acids Research·Nathan Wong, Xiaowei Wang
Dec 30, 2014·Clinical Biochemistry·Shaymaa M M Yahya, Ghada H Elsayed
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 11, 2016·Cancer Science·Akihiko ShimomuraTakahiro Ochiya
Oct 21, 2017·Scientific Reports·Diana Drago-GarcíaEnrique Hernández-Lemus

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Citations

Oct 11, 2019·Cells·Mohammad Mijanur RahmanTrygve O Tollefsbol
Feb 16, 2020·Molecular Cancer·Ranjana K KanchanMohd W Nasser
Jan 8, 2020·Cancers·Carla Solé, Charles H Lawrie
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
May 16, 2019·Experimental and Therapeutic Medicine·Huixiang YaoJinshui Zhu
Dec 30, 2020·Cancer Cell International·Yueyue YangJiayi Wang
Jan 24, 2019·Cancer Letters·Marjorie GournayRémy Pedeux
Sep 10, 2021·Cellular and Molecular Life Sciences : CMLS·Qiu-Luo LiuZong-Guang Zhou

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Methods Mentioned

BETA
electrophoresis
transfection
Assay
ELISA
xenograft
flow cytometry

Software Mentioned

cBioportal
SPSS

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