Tumor promotion studies in rat tracheal epithelium

Environmental Health Perspectives
V SteeleS B Pai

Abstract

The tracheal epithelium of the Fischer 344 rat is histologically very similar to that of the human bronchus. Also, carcinomas of tracheal origin in F-344 rats are similar in morphology to human bronchogenic carcinomas. Tumor promotion in rat tracheal epithelium was studied by using two model systems. The first is a heterotopic transplant system in which rat tracheas are implanted subcutaneously on the backs of isogenic recipents. In the first system, the epithelium was topically exposed to pellets containing 7,12-dimethylbenz(a)anthracene (DMBA), used as the initiating agent, followed by pellets containing the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), the promoting agent. After 98 weeks, a three- to fourfold increase in the percentage of tracheas having malignant tumors was seen in tracheal transplants receiving both DMBA and TPA compared to DMBA alone. Exposure of the tracheal grafts to TPA alone resulted in epithelial hyperplasia and inflammation, but no dysplastic lesions. The second system is an organ culture-cell culture system in which small pieces of trachea are grown in organ culture, then epithelial cells are grown from these pieces as primary cell cultures. The organ cultures were exposed to the direct...Continue Reading

References

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