Tumor protease-activated, pore-forming toxins from a combinatorial library

Nature Biotechnology
R G PanchalH Bayley

Abstract

We describe a library of two-chain molecular complementation mutants of staphylococcal alpha-hemolysin that features a combinatorial cassette encoding thousands of protease recognition sites in the central pore-forming domain. The cassette is flanked by a peptide extension that inactivates the protein. We screened the library to identify alpha-hemolysins that are highly susceptible to activation by cathepsin B, a protease that is secreted by certain metastatic tumor cells. Toxins obtained by this procedure should be useful for the permeabilization of malignant cells thereby leading directly to cell death or permitting destruction of the cells with drugs that are normally membrane impermeant.

References

Jul 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L M Matrisian
May 1, 1991·The Biochemical Journal·H E KhouriR Ménard
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·C T ChungR H Miller
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S YemulH Bayley
Apr 1, 1973·The Biochemical Journal·A J Barrett
Jan 1, 1981·Methods in Enzymology·A J Barrett, H Kirschke
Jun 5, 1981·Science·B F SloaneK V Honn
Sep 29, 1995·The Journal of Biological Chemistry·R G Panchal, H Bayley
Jul 28, 1995·The Journal of Biological Chemistry·A TaralpS Hasnain
Jun 15, 1995·Clinica Chimica Acta; International Journal of Clinical Chemistry·M K Schwartz
Jun 1, 1994·Trends in Biotechnology·W M Pardridge
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·J E GouauxH Bayley
May 1, 1994·Protein Engineering·B WalkerH Bayley
Jan 1, 1994·Protein Engineering·B Walker, H Bayley
Feb 1, 1994·Biochemical Society Transactions·D KepplerB Sordat
Dec 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·W G Stetler-StevensonD E Kleiner
Aug 9, 1993·FEBS Letters·R MénardA C Storer

❮ Previous
Next ❯

Citations

Nov 25, 2004·Progress in Biophysics and Molecular Biology·Michael W Parker, Susanne C Feil
Feb 17, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·J E KoblinskiB F Sloane
Oct 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·G MenestrinaG Prévost
Oct 8, 1999·Pharmacology & Therapeutics·G M Dubowchik, M A Walker
Sep 1, 2000·Advanced Drug Delivery Reviews·F NilssonD Neri
Dec 18, 2001·Advanced Drug Delivery Reviews·P Meers
Jun 6, 2000·Advanced Drug Delivery Reviews·O V GerasimovD H Thompson
Mar 4, 2000·Advanced Drug Delivery Reviews·S M Moghimi, A R Rajabi-Siahboomi
Feb 27, 1999·Current Opinion in Biotechnology·H Bayley
Aug 1, 1997·Current Opinion in Structural Biology·H Zhao, F H Arnold
Apr 13, 2000·Bioorganic & Medicinal Chemistry Letters·N SchaschkeL Moroder
Oct 13, 2012·Chemical Reviews·Ekaterina M Nestorovich, Sergey M Bezrukov
Jul 1, 1996·Nature Biotechnology·B F Sloane
Sep 23, 2006·Nature Reviews. Cancer·Mona Mostafa Mohamed, Bonnie F Sloane
Feb 18, 2011·The Journal of Biological Chemistry·Anne F HammersteinHagan Bayley
Jul 1, 1999·Protein Science : a Publication of the Protein Society·S CheleyH Bayley
Aug 26, 2014·Toxins·Philip A Gurnev, Ekaterina M Nestorovich
Aug 13, 2015·Journal of Biological Physics·Jirasak Wong-Ekkabut, Mikko Karttunen
Jun 22, 2010·Current Opinion in Biotechnology·Sheereen MajdMichael Mayer
Jun 1, 2010·Biomaterials·François HallouardThierry Vandamme
Mar 10, 2009·Toxicon : Official Journal of the International Society on Toxinology·Katarina Crnigoj KristanGregor Anderluh
Mar 10, 2009·Toxicon : Official Journal of the International Society on Toxinology·Mayra TejucaMauro Dalla Serra
Mar 4, 1998·Biochemical Pharmacology·R G Panchal
Dec 19, 2003·Biochemical and Biophysical Research Communications·Xiaoyu JiangAnlong Xu
Sep 17, 2004·Molecular Membrane Biology·Hagan Bayley, Lakmal Jayasinghe
Jul 21, 2009·Biochemical Society Transactions·Paul Curnow
Oct 9, 2002·The Journal of Peptide Research : Official Journal of the American Peptide Society·J A WerkmeisterD E Rivett
Jan 31, 2018·Proceedings of the National Academy of Sciences of the United States of America·Wei TianHammad Naveed
Apr 12, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Thomas WilkopQuan Cheng
Jul 4, 1998·Biochimica Et Biophysica Acta·C C PakP Meers
Jul 1, 1997·Chemistry & Biology·O BrahaH Bayley
Aug 14, 2012·Journal of Chemical Theory and Computation·Jirasak Wong-Ekkabut, Mikko Karttunen
Oct 3, 2018·ACS Chemical Biology·Natalie L MutterGiovanni Maglia
May 2, 2019·ACS Central Science·Sunwoo KooHagan Bayley
Aug 31, 2019·Journal of the American Chemical Society·Natalie L MutterGiovanni Maglia

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.