Tumor-specific delivery of therapeutic siRNAs by anti-EGFR immunonanoparticles

International Journal of Nanomedicine
Jung Seok KimYong Serk Park

Abstract

Efficient target-specific siRNA delivery has always been a primary concern in the field of siRNA clinical application. In this study, four different types of anti-epidermal growth factor receptor (EGFR) antibody-conjugated immunonanoparticles were prepared and tested for cancer cell-targeted therapeutic siRNA delivery. The prepared nanoparticles encapsulating siRNAs were character-ized by gel retardation and particle analysis using a Zetasizer. In vitro transfection and reduction of target genes, vimentin and JAK3, were determined using quantitative reverse transcription polymerase chain reaction. In vivo tumor targeting and antitumoral efficacies of the nanoparticles were evaluated in mice carrying tumors. Among these immunonanoparticles, anti-EGFR immunolipoplexes and immunoviroplexes exhibited remarkable cell binding and siRNA delivery to EGFR-expressing tumor cells compared to immunoliposomes and immunovirosomes. Especially, the anti-EGFR immunoviroplexes exhibited the most efficient siRNA transfection to target tumor cells. Therefore, antitumoral vimentin and Janus kinase-3 siRNAs were loaded in the anti-EGFR immunolipoplexes and immunoviroplexes, which were tested in mice carrying SK-OV-3 tumor xenografts. In fact, the th...Continue Reading

Citations

Jan 15, 2021·Molecular Pharmaceutics·Oleksandr ZavoiuraKerstin Jahn-Hofmann

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Methods Mentioned

BETA
gene knockdown
transfection
xenografts
PCR
fluorescence microscopy
gel filtration
flow cytometry
protein assay

Software Mentioned

Quantity One

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