PMID: 9419450Jan 7, 1998Paper

Tumor-specific immune response: current in vitro analyses may not reflect the in vivo immune status

Critical Reviews in Immunology
F FaureP Kourilsky

Abstract

Although our knowledge and understanding of tumor-specific cytotoxic T lymphocytes (CTL) have expanded considerably, the long-term work needed to assay CTL has precluded their analysis in large numbers of patients. Moreover, in vitro culture steps may introduce major biases. New approaches to identify tumor-specific CTL clones would be helpful. As a means to describe the in situ immune status by T-cell repertoire analysis, we developed the Immunoscope approach, a PCR-based method that allows us to determine the spectra of CDR3 lengths of the TCR chains displayed by complex populations of T cells. We review here some of our data about melanoma. Tumor-infiltrating lymphocytes of a melanoma patient were analyzed by different means and melanoma-specific T-cell clones were derived. Two categories of tumor-specific CD8+ CTL clones were derived from the infiltrate of a tumor-proximal invaded lymph node. The majority of T-cell clones specifically lyse the autologous tumor cell lines and predominantly recognize the HLA-A2/MART-1(27-35) peptide complex. The in vivo representativity of such CTL was assessed by the immunoscope technology. Among three MART-1-specific clones, none was detectable in situ. The other kind of tumor-specific CD8+...Continue Reading

Citations

Dec 25, 2003·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·M De AmiciR Moratti
Apr 10, 2004·Journal of Translational Medicine·Per Thor StratenJürgen C Becker
Aug 24, 2012·PloS One·Keith R MillerEdward J Collins
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·P T StratenJ C Becker
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·W HerrW J Storkus
Feb 13, 2016·Frontiers in Cellular Neuroscience·Silvia Sánchez-Ramón, Florence Faure
Sep 1, 1999·The Journal of Investigative Dermatology·P MusetteG Gachelin
Mar 29, 2002·The Journal of Investigative Dermatology·Lars Østergaard PedersenJürgen C Becker
Apr 4, 2000·The Journal of Investigative Dermatology·P thor StratenJ C Becker
Dec 14, 1999·The Journal of Investigative Dermatology·J C BeckerP T Straten
Apr 20, 2000·The Journal of Investigative Dermatology·C S VetterJ C Becker
Jul 8, 2008·Plant Physiology and Biochemistry : PPB·Eun Kyung Cho, Mihyang Kim
Sep 10, 2005·International Journal of Cancer. Journal International Du Cancer·Nathalie T JonckerOlivier Lantz
Jul 20, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·P thor StratenP Guldberg
Aug 5, 2000·International Journal of Cancer. Journal International Du Cancer·A LetschC Scheibenbogen
May 16, 2006·Journal of Immunotherapy·Kimberly Shafer-WeaverAnatoli Malyguine
Mar 18, 2015·Current Treatment Options in Oncology·Neal DharmadhikariHoward L Kaufman
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G L Beatty, Y Paterson
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J N BlattmanJ D Altman
Oct 3, 1999·International Journal of Cancer. Journal International Du Cancer·B CochloviusM Zöller
Dec 18, 2001·European Journal of Immunology·I M BelyakovJ A Berzofsky

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