Tumor suppressor RASSF1A is a microtubule-binding protein that stabilizes microtubules and induces G2/M arrest

Oncogene
R RongY Huang

Abstract

RASSF1A is a putative tumor suppressor gene that is inactivated in a variety of human tumors. Expression of exogenous RASSF1A has been shown to inhibit tumor growth in vitro and in animals. However, the molecular mechanisms by which RASSF1A mediates its tumor suppressive effects remain to be elucidated. Here, we report that RASSF1A is a microtubule-binding protein that interacts with and stabilizes microtubules. We have identified the RASSF1A region harboring a basic domain that appears to mediate the interactions between RASSF1A and microtubules. The basic domain-containing RASSF1C isoform also interacts with and stabilizes microtubules. We further show that in addition to G1 arrest, RASSF1A promotes growth arrest in the G2/M phase of the cell cycle and endogenous RASSF1A also interacts with microtubules. Based on our results, we propose that RASSF1A may mediate its tumor suppressive effects by inducing growth arrest in the G1 and G2/M phases. Together, these results provide important new insights into the molecular mechanisms by which this novel tumor suppressor mediates its biological effects.

References

Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Aug 9, 1991·Science·K W KinzlerD McKechnie
Dec 1, 1990·The Journal of Cell Biology·I Irminger-FingerL S Goldstein
Jun 1, 1987·Applied Biochemistry and Biotechnology·R J Linhardt
Aug 16, 1994·Biochemistry·N GustkeE Mandelkow
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·C BoscR L Margolis
Oct 18, 1996·Cell·K W Kinzler, B Vogelstein
Dec 1, 1996·Current Opinion in Cell Biology·A D Rudner, A W Murray
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·L BerruetaB E Bierer
Feb 27, 1999·Current Opinion in Cell Biology·L Cassimeris
Mar 11, 1999·Current Opinion in Genetics & Development·A Amon
Feb 19, 2000·Current Opinion in Cell Biology·C E Walczak
Oct 14, 2000·Annual Review of Cell and Developmental Biology·K H Downing
Dec 19, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J SzalayR Vazeos
Apr 3, 2001·Nature Cell Biology·K B KaplanI S Näthke
May 3, 2001·Journal of the National Cancer Institute·D G BurbeeJ D Minna
May 15, 2001·Acta Physiologica Scandinavica·A BäckerH J Kramer
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·K DreijerinkE R Zabarovsky
Jul 10, 2001·Annual Review of Biophysics and Biomolecular Structure·E Nogales
Sep 21, 2001·The Journal of Cell Biology·Y Mimori-Kiyosue, S Tsukita
Oct 23, 2001·International Journal of Cancer. Journal International Du Cancer·J H YoonG P Pfeifer
Apr 16, 2002·The British Journal of Surgery·S P Balasubramanian
Apr 17, 2002·Cell·Soni L Shimoda, Frank Solomon
Apr 27, 2002·Nature·Julie D R Reimann, Peter K Jackson
May 4, 2002·Nature Reviews. Molecular Cell Biology·Mariann Bienz
May 25, 2002·Molecular and Cellular Biology·Latha ShivakumarMichael A White
Jun 18, 2002·Current Opinion in Cell Biology·Tomoyuki U Tanaka
Oct 3, 2002·Nature Cell Biology·Snezhana Oliferenko, Mohan K Balasubramanian
Nov 7, 2003·Oncogene·Limin LiuGerd P Pfeifer
Dec 10, 2003·Oncogene·Kapil N Bhalla
Jan 27, 2004·Nature Cell Biology·Min Sup SongDae-Sik Lim
Aug 25, 2004·Current Biology : CB·Fred Kippert, Dietlind L Gerloff

❮ Previous
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Citations

Oct 13, 2009·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Geqiong XiaoGang Wu
Apr 7, 2011·Protein & Cell·Sunil K VermaPeter J Parker
Aug 21, 2013·Advances in Biological Regulation·Jia Jia Chan, Matilda Katan
Oct 18, 2005·Lancet·David M Jackman, Bruce E Johnson
Sep 2, 2005·Molecular and Cellular Biology·L van der WeydenA Bradley
Jan 31, 2014·Molecular Biology of the Cell·Christopher ArnetteIrina Kaverina
Jun 16, 2012·Molecular Biology International·Marilyn GordonShairaz Baksh
Aug 19, 2014·The Journal of Clinical Investigation·Chester E ChamberlainMichael S German
Jul 19, 2013·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Hae-Yun JungYeul Hong Kim
Jun 7, 2011·Oncogene·S GiovinazziA M Ishov
Apr 21, 2009·Expert Review of Anticancer Therapy·Camilla L ChristensenHans S Poulsen
Mar 13, 2014·FEBS Letters·Natalia VolodkoShairaz Baksh
Jun 3, 2008·FEBS Letters·Sunil K VermaPeter J Parker
Apr 25, 2012·Seminars in Cell & Developmental Biology·Alexander Hergovich, Brian A Hemmings
Apr 10, 2009·International Journal of Cancer. Journal International Du Cancer·Shui Ping TuBenjamin Chun-Yu Wong
Mar 28, 2009·Environmental and Molecular Mutagenesis·Junichiro Yasunaga, Kuan-Teh Jeang
Jun 27, 2012·International Journal of Cancer. Journal International Du Cancer·Maria Sofia FernandesRaquel Seruca
Aug 19, 2007·Biochemical and Biophysical Research Communications·Shankar JagadeeshPartha P Banerjee
May 12, 2012·Molecular Biology International·Claudia DittfeldReinhard H Dammann
Apr 7, 2009·Biochimica Et Biophysica Acta·Antje M RichterReinhard H Dammann
Aug 19, 2007·Biochimica Et Biophysica Acta·Louise van der Weyden, David J Adams
May 15, 2007·Genomics·Helena HaugeHans-Christian Aasheim
Oct 21, 2015·Biochemical and Biophysical Research Communications·Nobuya SakaiKatsushi Shibata
Jul 22, 2019·Cancers·Giorgio MalpeliClaudio Bassi
Dec 6, 2019·Cell Death & Disease·Fatéméh DuboisGuénaëlle Levallet
Oct 14, 2019·Breast Cancer Research and Treatment·Sylvie Rodrigues-FerreiraClara Nahmias
Jun 14, 2005·Biochemistry. Biokhimii︠a︡·G P Pfeifer, R Dammann
Oct 12, 2018·Cell Communication and Signaling : CCS·Fatéméh DuboisGuénaëlle Levallet

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