Tumor suppressor role of miR-133a in gastric cancer by repressing IGF1R

World Journal of Gastroenterology : WJG
Yu GongLi-Ming Tang

Abstract

To investigate the function and mechanism of miR-133a in gastric cancer (GC) and its relationship with clinicopathological characteristics of GC. A total of 105 GC patients who underwent surgical resection as primary treatment were selected for this study. Real-time quantitative reverse transcriptase polymerase chain (qRT-PCR) was used to examine the expression levels of miR-133a in human GC and adjacent non-tumor tissues, as well as in GC cell lines (SGC-7901, BGC-823, MGC-803, and AGS) and a human gastric mucosal epithelial cell line (GES-1). The biological role of miRNA (miR)-133a was assessed in the GC cell lines using MTT, apoptosis, migration and invasion, and colony formation assays, and xenograft tumorigenesis. qRT-PCR and western blot analyses were used to evaluate the potential target gene expression of miR-133a. Pearson's correlation was calculated to evaluate the correlation between miR-133a and insulin-like growth factor 1 receptor (IGF1R) expression. The regulation of IGF1R by miR-133a was verified using the luciferase reporter assay. In 80% of the 105 GC patients, the mean expression of miR-133a was significantly downregulated in tumor tissues compared with adjacent normal tissues (1.215 ± 0.1477 vs 3.093 ± 0.410...Continue Reading

References

Nov 22, 2005·Lancet·Goodarz DanaeiUNKNOWN Comparative Risk Assessment collaborating group (Cancers)
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Jul 13, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Hermien HartogWinette T A van der Graaf
Sep 27, 2007·Molecular Cancer·William C S Cho
Jan 30, 2009·Journal of Gastroenterology and Hepatology·Junming GuoYanjun Wang
Feb 19, 2009·British Journal of Cancer·T MurayamaK Sugihara
Nov 26, 2009·Biochimica Et Biophysica Acta·William C S Cho
Feb 10, 2011·British Journal of Cancer·H YoshinoM Nakagawa
Nov 1, 2009·Molecular Medicine Reports·Yu YaoChen Huang
May 3, 2011·Current Genomics·Leigh-Ann Macfarlane, Paul R Murphy
Jun 1, 2013·Molecular Cancer Research : MCR·Yujuan DongJun Yu
Oct 16, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jinling GuoGe Lou
Jul 2, 2014·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y J Xu, Y Fan
Jul 8, 2014·BioMed Research International·Norahayu Othman, Noor Hasima Nagoor
Jul 9, 2014·International Journal of Molecular Sciences·Hui XiaXiangyang Chu

❮ Previous
Next ❯

Citations

Jul 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wei ZhangYahui Liu
Mar 16, 2017·Journal of Cellular Biochemistry·Wei WeiLing Tang
May 4, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wenfeng LiYunsheng Xu
Jul 28, 2019·Pathogens and Disease·Narges DastmalchiSeyed Mahdi Banan Khojasteh
Jun 7, 2019·Journal of Cellular Physiology·Bahman YousefiMajid Eslami
Jul 11, 2020·Indian Journal of Clinical Biochemistry : IJCB·Zohreh HojatiHossein Khanahmad
Nov 22, 2018·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Enxu BiJingtao Wang
Aug 11, 2020·Human Cell·Yubin FengFeihu Chen
Nov 17, 2017·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Suzanne DufresneDarren R Brenner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis