Tumor susceptibility gene 101 (TSG101) is a novel binding-partner for the class II Rab11-FIPs.

PloS One
Conor P HorganMary W McCaffrey

Abstract

The Rab11-FIPs (Rab11-family interacting proteins; henceforth, FIPs) are a family of Rab11a/Rab11b/Rab25 GTPase effector proteins implicated in an assortment of intracellular trafficking processes. Through proteomic screening, we have identified TSG101 (tumor susceptibility gene 101), a component of the ESCRT-I (endosomal sorting complex required for transport) complex, as a novel FIP4-binding protein, which we find can also bind FIP3. We show that α-helical coiled-coil regions of both TSG101 and FIP4 mediate the interaction with the cognate protein, and that point mutations in the coiled-coil regions of both TSG101 and FIP4 abrogate the interaction. We find that expression of TSG101 and FIP4 mutants cause cytokinesis defects, but that the TSG101-FIP4 interaction is not required for localisation of TSG101 to the midbody/Flemming body during abscission. Together, these data suggest functional overlap between Rab11-controlled processes and components of the ESCRT pathway.

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Citations

Oct 4, 2012·Nature Cell Biology·Arnaud Echard
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Methods Mentioned

BETA
GTPase
GTPases
two-hybrid
confocal microscopy
Co-immunoprecipitation
transfection
PCR
Infrared Imaging
fluorescence microscopy

Software Mentioned

Zeiss ZEN Light Edition
Odyssey Infrared Imaging Application Software ( LI - COR )
Zeiss LSM Image Browser
Adobe Illustrator
Excel

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