Tumor Targeting via Sialic Acid: [68 Ga]DOTA-en-pba as a New Tool for Molecular Imaging of Cancer with PET

Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging
Charalambos TsoukalasPenelope Bouziotis

Abstract

The aim of this study was to demonstrate the potential of Ga-68-labeled macrocycle (DOTA-en-pba) conjugated with phenylboronic vector for tumor recognition by positron emission tomography (PET), based on targeting of the overexpressed sialic acid (Sia). The imaging reporter DOTA-en-pba was synthesized and labeled with Ga-68 at high efficiency. Cell binding assay on Mel-C and B16-F10 melanoma cells was used to evaluate melanin production and Sia overexpression to determine the best model for demonstrating the capability of [68Ga]DOTA-en-pba to recognize tumors. The in vivo PET imaging was done with B16-F10 tumor-bearing SCID mice injected with [68Ga]DOTA-en-pba intravenously. Tumor, blood, and urine metabolites were assessed to evaluate the presence of a targeting agent. The affinity of [68Ga]DOTA-en-pba to Sia was demonstrated on B16-F10 melanoma cells, after the production of melanin as well as Sia overexpression was proved to be up to four times higher in this cell line compared to that in Mel-C cells. Biodistribution studies in B16-F10 tumor-bearing SCID mice showed blood clearance at the time points studied, while uptake in the tumor peaked at 60 min post-injection (6.36 ± 2.41 % ID/g). The acquired PET images were in accor...Continue Reading

References

May 1, 1993·Clinical & Experimental Metastasis·M KimotoS Kanegasaki
Jun 26, 2001·Glycoconjugate Journal·R Schauer
Feb 17, 2007·Contrast Media & Molecular Imaging·Kristina DjanashviliJoop A Peters
Sep 18, 2007·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Konstantin P ZhernosekovFrank Rösch
Apr 3, 2008·Contrast Media & Molecular Imaging·Melpomeni FaniHelmut R Maecke
Jul 17, 2008·The British Journal of Radiology·A M Blamire
Mar 17, 2010·Carbohydrate Research·Aurélie CazetPhilippe Delannoy
Mar 30, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Frank Rösch, Richard P Baum
Sep 24, 2011·International Journal of Molecular Imaging·Ivan Martinez-DunckerCarlos Martinez-Duncker
Jan 5, 2012·Current Pharmaceutical Biotechnology·Sijumon KunjachanTwan Lammers
Feb 24, 2012·Molecular Oncology·N Lynn Henry, Daniel F Hayes
Mar 6, 2012·European Journal of Nuclear Medicine and Molecular Imaging·Clemens DecristoforoAlfons Verbruggen
Mar 6, 2012·European Journal of Nuclear Medicine and Molecular Imaging·Melpomeni Fani, Helmut R Maecke
Mar 6, 2012·European Journal of Nuclear Medicine and Molecular Imaging·Adil AL-Nahhas, Stefano Fanti
Apr 3, 2012·Current Cancer Drug Targets·R A FalconerL H Patterson
Mar 13, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·David A MankoffAmy S Clark
Jan 1, 2013·Cells·Sylvain JulienPhilippe Delannoy
Aug 15, 2014·Advanced Drug Delivery Reviews·Randall ToyEfstathios Karathanasis
Feb 13, 2015·European Journal of Nuclear Medicine and Molecular Imaging·Monique R BernsenMarion de Jong
Aug 21, 2015·Nature Reviews. Cancer·Salomé S Pinho, Celso A Reis
Nov 1, 2015·Glycobiology·Oliver M T Pearce, Heinz Läubli

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Citations

Oct 2, 2020·Organic & Biomolecular Chemistry·Jonathan MartinelliKristina Djanashvili
Mar 17, 2019·Pharmacology & Therapeutics·Asurayya WorredeAlessandro Fatatis
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jonathan MartinelliKristina Djanashvili

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