Tumor vascular permeability factor stimulates endothelial cell growth and angiogenesis.

The Journal of Clinical Investigation
D T ConnollyJ Feder

Abstract

Vascular permeability factor (VPF) is an Mr 40-kD protein that has been purified from the conditioned medium of guinea pig line 10 tumor cells grown in vitro, and increases fluid permeability from blood vessels when injected intradermally. Addition of VPF to cultures of vascular endothelial cells in vitro unexpectedly stimulated cellular proliferation. VPF promoted the growth of new blood vessels when administered into healing rabbit bone grafts or rat corneas. The identity of the growth factor activity with VPF was established in four ways: (a) the molecular weight of the activity in preparative SDS-PAGE was the same as VPF (Mr approximately 40 kD); (b) multiple isoforms (pI greater than or equal to 8) for both VPF and the growth-promoting activity were observed; (c) a single, unique NH2-terminal amino acid sequence was obtained; (d) both growth factor and permeability-enhancing activities were immunoadsorbed using antipeptide IgG that recognized the amino terminus of VPF. Furthermore, 125I-VPF was shown to bind specifically and with high affinity to endothelial cells in vitro and could be chemically cross-linked to a high-molecular weight cell surface receptor, thus demonstrating a mechanism whereby VPF can interact directly ...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·G Carpenter, S Cohen
May 1, 1986·Microvascular Research·L E Gerlowski, R K Jain
Jan 1, 1987·Nouvelle Revue Française D'hématologie·A Camez, G Tobelem
May 1, 1988·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·B L EppleyJ Feder
Jan 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H Hoshi, W L McKeehan
Jul 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A Knedler, R G Ham
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M KlagsbrunJ A Smith
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K A ThomasS Fitzpatrick
Nov 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D GospodarowiczP Böhlent

❮ Previous
Next ❯

Citations

Jul 1, 1996·The Journal of Pathology·S B FoxA L Harris
Nov 14, 1997·International Journal of Cancer. Journal International Du Cancer·K MaedaM Sowa
Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·H DingA Guha
Feb 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J WiltingB Christ
Nov 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·G BreierW Risau
Apr 23, 2002·Journal of Cellular Biochemistry·Pascal N BernatchezMartin G Sirois
May 26, 1998·Biochemical and Biophysical Research Communications·K IgarashiI Uno
Feb 19, 2000·Biochemical and Biophysical Research Communications·Y SugishitaT Takahashi
Apr 25, 2000·Biochemical and Biophysical Research Communications·R RamakrishnanA Rathinavelu
Jun 1, 1996·Cancer Metastasis Reviews·K P Claffey, G S Robinson
Jun 1, 1996·Cancer Metastasis Reviews·K H Plate
Jan 1, 1995·Breast Cancer Research and Treatment·N Ferrara
Jan 1, 1995·Breast Cancer Research and Treatment·W KolchD Marmé
May 1, 1997·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·X YiM Uyama
Feb 9, 2006·Cellular and Molecular Life Sciences : CMLS·S Cébe-SuarezK Ballmer-Hofer
Dec 15, 2004·Der Anaesthesist·S FischerG F Karliczek
Sep 1, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Tae-Wook ChungCheorl-Ho Kim
Sep 19, 2009·Abdominal Imaging·Yan ChenChunwu Zhou
Nov 7, 2006·Cancer Immunology, Immunotherapy : CII·Kousaku MimuraHideki Fujii
Jan 17, 2012·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Muhittin BelirgenM Memet Ozek
Feb 24, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Gholam A PeymanMandi D Conway
Aug 23, 2012·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Young Jung RohJung Il Moon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.