Tumoricidal activity of endothelial cells. Inhibition of endothelial nitric oxide production abrogates tumor cytotoxicity induced by hepatic sinusoidal endothelium in response to B16 melanoma adhesion in vitro

The Journal of Biological Chemistry
J CarreteroJ M Estrela

Abstract

The mechanism of NO- and H(2)O(2)-induced tumor cytotoxicity was examined during B16 melanoma (B16M) adhesion to the hepatic sinusoidal endothelium (HSE) in vitro. We used endothelial nitric-oxide synthetase gene disruption and N(G)-nitro-l-arginine methyl ester-induced inhibition of nitric-oxide synthetase activity to study the effect of HSE-derived NO on B16M cell viability. Extracellular H(2)O(2) was removed by exogenous catalase. H(2)O(2) was not cytotoxic in the absence of NO. However, NO-induced tumor cytotoxicity was increased by H(2)O(2) due to the formation of potent oxidants, likely ( small middle dot)OH and (-)OONO radicals, via a trace metal-dependent process. B16M cells cultured to low density (LD cells), with high GSH content, were more resistant to NO and H(2)O(2) than B16M cells cultured to high density (HD cells; with approximately 25% of the GSH content found in LD cells). Resistance of LD cells decreased using buthionine sulfoximine, a specific GSH synthesis inhibitor, whereas resistance increased in HD cells using GSH ester, which delivers free intracellular GSH. Because NO and H(2)O(2) were particularly cytotoxic in HD cells, we investigated the enzyme activities that degrade H(2)O(2). NO and H(2)O(2) cause...Continue Reading

References

Jul 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T W McCloskeyD L Laskin
Nov 1, 1992·Chemical Research in Toxicology·W H KoppenolJ S Beckman
Nov 1, 1992·Cancer Metastasis Reviews·R LafrenieF W Orr
Jan 1, 1990·Advances in Cancer Research·L Weiss
Nov 1, 1990·British Journal of Pharmacology·D D ReesS Moncada
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J Mårtensson, A Meister
Nov 30, 1988·Biochemical and Biophysical Research Communications·J B HibbsE M Rachlin
Jan 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L WeissK V Honn
Jan 1, 1984·Methods in Enzymology·L Flohé, F Otting
Jan 1, 1984·Methods in Enzymology·L Flohé, W A Günzler
Jan 1, 1984·Methods in Enzymology·H Aebi
Nov 24, 1995·The Journal of Biological Chemistry·V G KharitonovV S Sharma
Jan 1, 1995·Annual Review of Biochemistry·I Fridovich
Nov 1, 1995·The Journal of Experimental Medicine·R PacelliJ B Mitchell
Jul 1, 1995·Clinical & Experimental Metastasis·P A AlbertssonB R Johansson
Apr 1, 1995·Free Radical Biology & Medicine·R H Burdon
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A WinkJ B Mitchell
May 1, 1993·The Journal of Experimental Medicine·P OroszD N Männel
May 5, 1993·Journal of the National Cancer Institute·A E EskenaziC N Frantz
May 1, 1996·Journal of Cellular Physiology·M J AnasagastiF Vidal-Vanaclocha
May 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L G BayónS Meijer
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·E G SheselyO Smithies
Jul 31, 1997·Nature·G MelinoA Finazzi-Agro
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M NishikawaM Inoue
May 15, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M J AnasagastiF Vidal-Vanaclocha
Jul 11, 1998·Carcinogenesis·D A WinkJ B Mitchell
Nov 24, 1999·Free Radical Biology & Medicine·H Sies
May 10, 2000·Nature Medicine·G Kroemer, J C Reed
Aug 6, 2000·Free Radical Biology & Medicine·H Sies, G E Arteel
Aug 6, 2000·Free Radical Biology & Medicine·N Hogg

❮ Previous
Next ❯

Citations

Jul 23, 2005·Clinical & Experimental Metastasis·J Milburn JessupL M Laguinge
Jul 20, 2002·Free Radical Biology & Medicine·Miguel AsensiJose M Estrela
Jan 12, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elena ObradorJosé M Estrela
Jan 13, 2016·Critical Reviews in Clinical Laboratory Sciences·José M EstrelaElena Obrador
May 14, 2008·Nitric Oxide : Biology and Chemistry·Angel OrtegaJosé M Estrela
Aug 5, 2003·Liver International : Official Journal of the International Association for the Study of the Liver·Katrien VekemansFilip Braet
Jun 15, 2013·Oxidative Medicine and Cellular Longevity·Nicola TraversoCinzia Domenicotti
Feb 17, 2010·Chemico-biological Interactions·Els L R SwennenPieter C Dagnelie
Aug 1, 2014·Cancer Letters·Huiwen ChengLi Zuo
Jan 1, 2010·Cancers·Angel L OrtegaJosé M Estrela
Jun 29, 2004·International Journal of Cancer. Journal International Du Cancer·John Milburn JessupPeter Thomas
Oct 24, 2018·Biological Chemistry·Elena ObradorJosé M Estrela
Mar 7, 2006·Critical Reviews in Clinical Laboratory Sciences·José M EstrelaElena Obrador
Oct 27, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Simone MocellinDonato Nitti
May 3, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Salvador MenaJosé M Estrela
May 20, 2020·Seminars in Cancer Biology·Elena ObradorJosé M Estrela
Jul 3, 2021·International Journal of Molecular Sciences·Elena ObradorJosé M Estrela
Aug 28, 2021·Biomedicines·Elena ObradorJosé M Estrela

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.