PMID: 6348087Jul 1, 1983Paper

Tumoricidal activity of human monocytes activated in vitro by free and liposome-encapsulated human lymphokines

The Journal of Clinical Investigation
E S KleinermanI J Fidler

Abstract

Human peripheral blood mononuclear cells from normal donors obtained by separation on a Percoll gradient were incubated with free or liposome-entrapped lymphokines produced from concanavalin A-stimulated lymphocytes and then were tested for cytotoxic activity against tumor cells. The treated monocytes lysed tumorigenic melanoma and glioblastoma target cells, but had no effect on three types of nontumorigenic target cells. The activation of monocytes to become tumoricidal was caused by macrophage-activating factor (MAF) and not by contamination with endotoxins, concanavalin A, or interferon. The endocytosis of liposomes containing MAF, but not of those containing control supernatants, led to the activation of cytotoxic properties in the monocytes. Activation by liposome-encapsulated MAF was very efficient and required less than 1/800th of the amount of free MAF necessary to achieve the same levels of cytotoxicity. Thus, the encapsulation of mitogen-induced MAF in liposomes could provide an effective approach for the activation of blood monocytes in situ.

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Citations

Sep 1, 1992·Journal of Surgical Oncology·H KonnoT Tadakuma
Feb 1, 1990·Cancer Metastasis Reviews·P W WhitworthI J Fidler
Aug 1, 1993·Cancer Immunology, Immunotherapy : CII·M MaedaE S Kleinerman
Jan 1, 1990·Cancer Immunology, Immunotherapy : CII·S SoneT Ogura
Jan 1, 1985·Springer Seminars in Immunopathology·R Nayar, I J Fidler
Jan 1, 1991·Biotherapy·C C Pak, I J Fidler
Apr 1, 1991·The Journal of Experimental Medicine·M R AldersonK H Grabstein
Oct 1, 1986·Journal of Interferon Research·I SaikiW C Koff
Apr 20, 2012·Immunotherapy·David A Christian, Christopher A Hunter
Mar 1, 1992·World Journal of Surgery·I J Fidler
Nov 1, 1993·Cancer Immunology, Immunotherapy : CII·T AsanoE S Kleinerman
Mar 31, 2012·European Journal of Clinical Pharmacology·Karthik VenkatakrishnanCristina Oliva
Feb 1, 1985·Immunological Investigations·J SenguptaB H Tom
Aug 10, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gaurav SharmaJeffrey W Smith
Jan 1, 1989·Critical Reviews in Oncology/hematology·A HagiwaraN Oku
Oct 10, 2015·Oncoimmunology·Marie-Agnès Doucey, Ioannis Xenarios
Apr 16, 2005·Drug Development and Industrial Pharmacy·Sara SeguraJuan Manuel Irache
Jan 1, 1996·The Veterinary Clinics of North America. Small Animal Practice·E G MacEwen, I D Kurzman
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Jan 1, 1987·Annals of the New York Academy of Sciences·R KirshG Poste
Jan 1, 1991·Biotherapy·G StormD J Crommelin

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