Tumorigenicity analysis of heterogeneous dental stem cells and its self-modification for chromosome instability

Cell Cycle
Zhaosong MengWeidong Tian

Abstract

Heterogeneity demonstrates that stem cells are constituted by several sub-clones in various differentiation states. The heterogeneous state is maintained by cross-talk among sub-clones, thereby ensuring stem cell adaption. In this study, we investigated the roles of heterogeneity on genetic stability. Three sub-clones (DF2, DF8 and DF18) were isolated from heterogeneous dental stem cells (DSCs), and were proved to be chromosome instability (CIN) after long term expansion. Cell apoptosis were not detected in sub-clones, which exhibited strong tumorigenesis tendency, coupled with weak expression of p53 and aberrant ultra-structure. However, 3 sub-clones did not overexpress tumor related markers or induce tumorigenesis in vivo. The mixed-culture study suggested that 3-clone-mixed culturing cells (DF1) presented apparent decrease in the ratio of aneuploidy. The screening experiment further proved that 3 sub-clones functioned separately in this modification procedure but only mixed culturing all 3 sub-clones, simulated heterogeneous microenvironment, could achieve complete modification. Additionally, osteogenesis capability of 3 sub-clones was partially influenced by CIN while DSCs still kept stronger osteogenesis than sub-clones. T...Continue Reading

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Citations

Mar 24, 2021·Stem Cell Research & Therapy·Jiayu ZhangWeihua Guo
Aug 18, 2021·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Yüksel KorkmazJames Deschner

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Methods Mentioned

BETA
Flow
xenograft
transfection
Fluorescence
Feature Extraction
Flow cytometry
Flow Cytometric
electrophoresis

Software Mentioned

Gene Spring
hclust
Graph
- Pad Prism
Quantity One
CXP
- Pad
Feature Extraction

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