PMID: 6989767May 15, 1980Paper

Tumorigenicity and in vitro characteristics of rat liver epithelial cells and their adenovirus-transformed derivatives

International Journal of Cancer. Journal International Du Cancer
C Paraskeva, P H Gallimore

Abstract

Cloned and uncloned epithelial cultures were established from the liver of a 3-week-old AS rat. These epithelial cultures were neither tumorigenic nor did they display anchorage-independent growth. One of the clones was cytogenetically normal after 53 in vitro passages (approximately 200 population doublings after cloning). Eight transformed lines were isolated from the liver epithelial cells after infection with adenovirus type 12 (Ad-12). Five of these produced typical Ad12 T-antigen, whereas three appeared to be T-antigen-negative. All were tumorigenic in newborn syngeneic rats. The T-antigen-positive transformed lines produced anaplastic-epithelial tumors, whereas the T-antigen-negative transformed lines produced adenocarcinomas. Although all the transformed lines were tumorigenic, some were fibronectin-positive while others produced no detectable fibronectin. The normal (untransformed) epithelial cells produced fibronectin. These results are interesting for two reasons: (1) there are relatively few reports of fibronectin on epithelial cells and (2) they emphasize the view that there is no absolute correlation between reduced fibronectin and tumorigenicity in transformed cells. The transformed lines displayed in vitro chara...Continue Reading

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Citations

Mar 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R K MallampalliG W Hunninghake
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P B FisherH S Ginsberg
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H C Isom, I Georgoff
Jan 1, 1982·The Journal of Cell Biology·D LouvardG Warren
Sep 1, 1996·Experimental Lung Research·M PasternackG W Hunninghake
Feb 15, 1984·International Journal of Cancer. Journal International Du Cancer·K W Brown, E K Parkinson
Nov 1, 1987·Journal of Virology·C D Woodworth, H C Isom
Jan 1, 1985·Biochimica Et Biophysica Acta·P E BrantonF L Graham

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