Tumour cell invasiveness and response to chemotherapeutics in adipocyte invested 3D engineered anisotropic collagen scaffolds.

Scientific Reports
Robert D HumeChristine J Watson

Abstract

Breast cancers are highly heterogeneous and their metastatic potential and response to therapeutic drugs is difficult to predict. A tool that could accurately gauge tumour invasiveness and drug response would provide a valuable addition to the oncologist's arsenal. We have developed a 3-dimensional (3D) culture model that recapitulates the stromal environment of breast cancers by generating anisotropic (directional) collagen scaffolds seeded with adipocytes and culturing tumour fragments therein. Analysis of tumour cell invasion in the presence of various therapeutic drugs, by immunofluorescence microscopy coupled with an optical clearing technique, demonstrated the utility of this approach in determining both the rate and capacity of tumour cells to migrate through the stroma while shedding light also on the mode of migration. Furthermore, the response of different murine mammary tumour types to chemotherapeutic drugs could be readily quantified.

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Citations

Jan 1, 2019·BMC Biomedical Engineering·Connor S MurphyMichaela R Reagan
Nov 22, 2020·Trends in Cancer·João RodriguesJai Prakash
Jun 27, 2021·Scientific Reports·Anna GustafssonGöran Landberg

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Datasets Mentioned

BETA
GM6001

Methods Mentioned

BETA
biopsy
transgenic
biopsies
fluorescence microscopy
confocal microscopy
dissection

Software Mentioned

Fiji
GraphPad
SIM
CUBIC
GraphPad Prism
ET
Excel

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