PMID: 8944615Nov 1, 1996Paper

Tumour heterogeneity of DNA cell cycle variables in breast cancer measured by flow cytometry

Journal of Clinical Pathology
E BergersJ P Baak

Abstract

Conflicting results have been reported concerning the prognostic value of DNA flow cytometric variables (DNA ploidy, DNA index, %S phase fraction) in breast cancer. Selection bias and differences in treatment may have contributed to these conflicting prognostic results. Differences in tissue processing, the number of nuclei measured, DNA histogram/cell cycle analysis, and intra-tumour heterogeneity may also have played a role. The aim of the present study was to assess intra-tumour heterogeneity of DNA flow cytometric variables in breast cancer. Fresh frozen specimens (n = 274) (0.3 x 0.3 x 0.3 cm) of 17 breast cancers and 167 slices, 50 microns thick, of 58 paraffin wax embedded blocks of 21 breast cancers were studied. All samples were prepared individually for DNA flow cytometry. DNA histograms were interpreted by semi-automated cell cycle analysis (MultiCycle) by two observers to avoid biased interpretation. An artificial averaged DNA histogram of each case was composed to simulate a sample prepared from whole tumour tissue. With regard to DNA ploidy, classified as diploid or aneuploid, the fresh frozen and paraffin wax embedded breast cancers showed intra-tumour heterogeneity in 53% and 38% of cases, respectively. For fres...Continue Reading

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Citations

Apr 3, 2002·Bulletin of Mathematical Biology·Alessandro BertuzziPaolo Ubezio
Dec 6, 2001·The American Journal of Pathology·J TorhorstG Sauter
Mar 6, 1999·Breast Cancer Research and Treatment·C R Wenger, G M Clark
May 8, 2013·Nature Reviews. Clinical Oncology·Roger P A'HernCharles Swanton
Jun 29, 2004·Journal of Clinical Pathology·P J van DiestJ P A Baak
Feb 19, 1999·Journal of Clinical Pathology·P J van DiestJ P Baak
Jun 29, 2001·Journal of Clinical Pathology·A E PintoJ Soares
Aug 26, 2010·The Journal of Comparative Neurology·Jurjen W WestraJerold Chun
Jun 20, 2012·Journal of Biophotonics·Susan M Daly, Martin J Leahy
Oct 10, 2013·BioMed Research International·Jamela BoderYrjö Collan
Apr 28, 2001·Evolution & Development·D S Thaler
Nov 27, 1999·British Journal of Cancer·L P MarsonJ M Dixon
Oct 25, 2011·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Dong Hwan KimJun Ah Lee
Jun 30, 2004·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D C ChhiengS Beeken
Feb 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yvette van HensbergenEpie Boven
May 21, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Aziza NassarCynthia Cohen

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