PMID: 9659158Jul 11, 1998Paper

Tumour necrosis factor-alpha enhances intraepithelial lymphocyte proliferation and migration

Gut
Ellen C Ebert

Abstract

Tumour necrosis factor alpha (TNF-alpha) is a proinflammatory cytokine found in abundance in diseased intestine. The T cell production of TNF-alpha and the impact of this cytokine on intestinal T cell proliferation, migration, and cytotoxicity were studied. Intestinal lymphocytes from normal jejunum were used. TNF-alpha production in culture supernates was measured by enzyme linked immunosorbent assay (ELISA). Lymphocyte proliferation was measured using 3H thymidine uptake; migration, using transwell chambers; and cytotoxicity of HT-29 colon cancer cells, using the chromium-51 release assay. TNF-alpha was produced mainly by the CD8+ T cells in the intraepithelial lymphocytes (IEL) and the CD4+ T cells in the lamina propria lymphocytes in response to CD2 stimulation: 478 (94) and 782 (136) pg/ml, respectively. TNF-alpha (1 ng/ml or greater) augmented proliferation of IEL in response to interleukin 2 (IL-2), IL-7, or antibody to CD3 due to increased activation that did not involve IL-2 production or receptor generation. Conversely, antibody to TNF-alpha reduced IEL proliferation in response to IL-2 or IL-7. TNF-alpha also induced calcium mobilisation and chemokinesis (by 2.8 (0.5) fold over spontaneous migration). TNF-alpha had n...Continue Reading

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Citations

Dec 30, 1999·Veterinary Immunology and Immunopathology·R Pabst, H J Rothkötter
Apr 7, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Richard S BlumbergSean P Colgan
May 23, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Yuan Qiu, Hua Yang
Oct 7, 2006·Current Opinion in Gastroenterology·E Isolauri
Jul 6, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Fuju ChangHarriet Deere
Feb 22, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·V A GuazzoneL Lustig
May 3, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K M JärvinenH Suomalainen
Mar 31, 2011·Histopathology·Mohammed NaimMohammed K A Sherwani
Sep 13, 2007·Immunopharmacology and Immunotoxicology·Edina NémethPéter Gálfi
Nov 3, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Karine VidalAnne Donnet-Hughes
Nov 1, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Naoki HosoeHiromasa Ishii
Dec 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yu ChenYong-Fu Xiao
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chikako WatanabeHiromasa Ishii
Mar 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Karin JöhrerRichard Greil

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