Tumour necrosis factor-alpha provokes upregulation of alpha2beta1 and alpha5beta1 integrins, and cell migration in OST osteosarcoma cells

Cell Biology International
A KawashimaI Nakanishi

Abstract

OST cells enhance the induction of matrix metalloproteinase (MMP)-9 by tumour necrosis factor (TNF)-alpha and the corresponding metastasis to lungs in vivo (Kawashima et al., 1994). We focused on the adhesive and migratory properties of OST cells, and investigated the expression of integrins in OST cells stimulated by TNFalpha in vitro. OST cells potentiated not only adhesion to the extracellular matrix (ECM) but also the migration on ECM. On competitive reverse transcription-polymerase chain reaction (RT-PCR) analyses, the amounts of alpha2 (4.9-fold), alpha5 (1.2-fold) and alpha(v) (4.9-fold) were upregulated by TNFalpha at the transcriptional level. Alpha-5 showed a slight increase by flow cytometry; however, alpha2 and alphav integrins remained unchanged at the protein level. Immunofluorescence study disclosed integrins of alpha2beta1 and alpha5beta1 were much clustered at cell processes by TNFalpha stimulation, probably related to increased cell adhesion and migration. Therefore, the upregulation of alpha2beta1 and alpha5beta1 integrins seems to contribute to tumour invasion and metastatic potential.

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Citations

Feb 1, 2007·DNA and Cell Biology·Maya Kansara, David M Thomas
Sep 3, 2014·Molecular Therapy. Nucleic Acids·Tomohiro FujiwaraTakahiro Ochiya
Mar 25, 2009·The British Journal of Dermatology·J P McFaddenL Fry
Mar 9, 2005·Homeopathy : the Journal of the Faculty of Homeopathy·D Y O SatoD de F Buchi
Oct 12, 2012·PloS One·Jeanine PignatelliChristopher E Turner
Aug 5, 2005·Journal of Cell Science·Roberto MontesanoFabio Carrozzino

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