Abstract
OST cells enhance the induction of matrix metalloproteinase (MMP)-9 by tumour necrosis factor (TNF)-alpha and the corresponding metastasis to lungs in vivo (Kawashima et al., 1994). We focused on the adhesive and migratory properties of OST cells, and investigated the expression of integrins in OST cells stimulated by TNFalpha in vitro. OST cells potentiated not only adhesion to the extracellular matrix (ECM) but also the migration on ECM. On competitive reverse transcription-polymerase chain reaction (RT-PCR) analyses, the amounts of alpha2 (4.9-fold), alpha5 (1.2-fold) and alpha(v) (4.9-fold) were upregulated by TNFalpha at the transcriptional level. Alpha-5 showed a slight increase by flow cytometry; however, alpha2 and alphav integrins remained unchanged at the protein level. Immunofluorescence study disclosed integrins of alpha2beta1 and alpha5beta1 were much clustered at cell processes by TNFalpha stimulation, probably related to increased cell adhesion and migration. Therefore, the upregulation of alpha2beta1 and alpha5beta1 integrins seems to contribute to tumour invasion and metastatic potential.
References
Apr 3, 1992·Cell·R O Hynes
Feb 20, 1991·International Journal of Cancer. Journal International Du Cancer·R MortariniG Parmiani
Mar 29, 1991·Science·B M ChanM E Hemler
Mar 9, 1990·Cell·F G Giancotti, E Ruoslahti
Sep 1, 1987·The Journal of Cell Biology·S DedharM D Pierschbacher
Jan 1, 1989·Connective Tissue Research·S DedharM D Pierschbacher
Jan 1, 1989·Annual Review of Immunology·B Beutler, A Cerami
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jan 6, 1995·The Journal of Biological Chemistry·T RiikonenJ Heino
May 1, 1995·The Journal of Cell Biology·P HuhtalaC H Damsky
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·M M ZutterY L Tsung
Jun 2, 1995·The Journal of Biological Chemistry·T RiikonenJ Heino
Jan 1, 1994·Virchows Archiv : an International Journal of Pathology·A KawashimaY Okada
May 8, 1993·International Journal of Cancer. Journal International Du Cancer·E H DanenC G Figdor
Oct 1, 1993·Journal of Cellular Physiology·H LarjavaJ Heino
May 31, 1996·Cell·P C BrooksD A Cheresh
Oct 1, 1996·Current Opinion in Cell Biology·J A Varner, D A Cheresh
Jun 13, 1998·The Journal of Biological Chemistry·B XieE Huberman
Citations
Feb 1, 2007·DNA and Cell Biology·Maya Kansara, David M Thomas
Sep 3, 2014·Molecular Therapy. Nucleic Acids·Tomohiro FujiwaraTakahiro Ochiya
Mar 25, 2009·The British Journal of Dermatology·J P McFaddenL Fry
Mar 9, 2005·Homeopathy : the Journal of the Faculty of Homeopathy·D Y O SatoD de F Buchi
Oct 12, 2012·PloS One·Jeanine PignatelliChristopher E Turner
Feb 7, 2003·Gene Therapy·A Brüning, I B Runnebaum
Aug 1, 2006·Oncogene·F de NigrisC Napoli
Dec 3, 2016·Oncology Letters·Jianxun Yang, Ning Wang
Dec 3, 2020·Cancer Management and Research·Daiqing WeiJuncai Liu
Aug 5, 2005·Journal of Cell Science·Roberto MontesanoFabio Carrozzino
May 16, 2003·Pathology, Research and Practice·Atsuhiro KawashimaYoshio Oda
Feb 25, 2010·Molecular Cancer·Ju-Fang LiuChih-Hsin Tang