Tumour overexpression of inducible nitric oxide synthase (iNOS) increases angiogenesis and may modulate the anti-tumour effects of the vascular disrupting agent ZD6126

Microvascular Research
Elizabeth R CullisSimon P Robinson

Abstract

Tumours derived from DLD-1 colon adenocarcinoma cells, transfected to either overexpress inducible nitric oxide synthase (clone iNOS-19) or with empty vector (pBAN2R), were utilised to test the hypothesis that tumour expression of iNOS (a) increases tumour angiogenesis and (b) modulates the anti-tumour activity of the vascular disrupting agent ZD6126. Overexpression of iNOS by clone iNOS-19 cells and murine xenografts was confirmed by the Griess assay and western blot analysis respectively. Clone iNOS-19 tumours grew more rapidly than pBAN2R tumours. Tumour perfusion, assessed by Hoechst 33342 uptake, was significantly greater in the clone iNOS-19 tumours (P < 0.001). A significant reduction in the perfusion of only the pBAN2R tumours, compared with control, was obtained 24 h after treatment with an intermediate dose of 100 mg/kg ZD6126 (P < 0.001), whereas 200 mg/kg significantly reduced the perfusion of both tumour types (P < 0.001). Whilst pBAN2R tumour necrosis increased in a dose-dependent manner, significant at 100 and 200 mg/kg ZD6126 (P < 0.05), intermediate doses did not induce a similar degree of necrosis in clone iNOS-19 tumours. A significant reduction in splenic perfusion was found 24 h after treatment with 100 mg/...Continue Reading

References

May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·D C JenkinsS Moncada
Jan 26, 1996·Biochemical and Biophysical Research Communications·H TrachtmanP C Singhal
Apr 29, 1998·Journal of the National Cancer Institute·O GalloM Ziche
Jul 11, 1998·Carcinogenesis·D A WinkJ B Mitchell
Dec 3, 2002·International Journal of Radiation Oncology, Biology, Physics·Dietmar W Siemann, Amyn M Rojiani
Dec 3, 2002·International Journal of Radiation Oncology, Biology, Physics·Peter D DavisSally A Hill
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Jun 17, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Helen L AndersonPatricia M Price
Oct 25, 2003·International Journal of Radiation Oncology, Biology, Physics·Michael R Horsman, Rumi Murata
Jun 3, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jeffrey L EvelhochJohn C Waterton
Jun 16, 2004·Cancer·Dietmar W SiemannMichael R Horsman
Jul 17, 2004·Neoplasia : an International Journal for Oncology Research·Vassiliki KostourouSimon P Robinson
Oct 29, 2004·Nature Reviews. Cancer·Carl-Henrik HeldinArne Ostman
Jan 22, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Sibylle LoiblManfred Kaufmann
Jun 2, 2005·Nature Reviews. Cancer·Gillian M TozerBruce C Baguley
Jun 22, 2005·Neoplasia : an International Journal for Oncology Research·Simon P RobinsonJohn C Waterton

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Citations

Jul 3, 2007·Cancer Metastasis Reviews·Debbie Liao, Randall S Johnson
Oct 22, 2013·BMC Complementary and Alternative Medicine·Sau Har LeeShamala Devi Sekaran
Aug 31, 2010·European Journal of Cell Biology·Swaraj SinhaSuvro Chatterjee
Jun 23, 2009·Fertility and Sterility·Anne-Sophie Van EyckMarie-Madeleine Dolmans
Jul 5, 2015·Microvascular Research·Ciara M McErleanSimon J Doran
Nov 21, 2007·Microvascular Research·Lynsey A HuxhamAndrew I Minchinton
Nov 23, 2007·International Journal of Cancer. Journal International Du Cancer·Simon P RobinsonArne Ostman
Sep 8, 2011·The British Journal of Dermatology·L C ChinG M Mitchell
Jun 21, 2012·IUBMB Life·Ka BianFerid Murad
Jan 13, 2016·International Journal of Cancer. Journal International Du Cancer·Efthymia PapaevangelouFranklyn A Howe
Dec 9, 2010·British Journal of Cancer·V KostourouS P Robinson
Apr 25, 2021·Nanomedicine : Nanotechnology, Biology, and Medicine·Antonia CharalambousP Louise Coletta

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