Tumour-specific cytotoxic T lymphocyte activity in Th2-type Sézary syndrome: its enhancement by interferon-gamma (IFN-gamma) and IL-12 and fluctuations in association with disease activity

Clinical and Experimental Immunology
N SeoM Takigawa

Abstract

Sézary syndrome (SzS) is the leukaemic variant of cutaneous T cell lymphoma (CTCL), whose malignant T cells are of the Th2 type in most cases. In this study we investigated the tumouricidal activity of cytotoxic T lymphocytes (CTL) present in peripheral blood of a patient with Th2-type SzS, focusing on the effect of IL-2, IFN-gamma and IL-12 on their cytotoxic activity, and the relationship between their lytic capacity and the patient's clinical course. At four different time points during a 2-month clinical period, CD4+CD7- Sézary cells and CD8+ cells were separated from the patient's circulating cells. CD8+ cells were cultured with chemically attenuated, purified Sézary cells in the presence of IL-2 to develop specific cytotoxicity. The CD8+ cells thus cultured exhibited lytic activity against autologous Sézary cells. Concomitant addition of IFN-gamma or IL-12 exerted a synergistic cytolytic effect with IL-2 on the tumour cells. Cytotoxicity inhibition studies using MoAbs revealed that the cytotoxicity operated in MHC class I-, CD8- and alphabeta T cell receptor-dependent manners. Furthermore, eight CD8+ T cell clones generated from cultured CD8+ cells exhibited a strong cytotoxicity against Sézary cells in an MHC class I-res...Continue Reading

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Citations

Apr 16, 2002·The British Journal of Dermatology·K ModschiedlerP von den Driesch
Oct 12, 2011·International Archives of Allergy and Immunology·Enrico ScalaAdriano Mari
Dec 11, 2002·Experimental Dermatology·Y TokuraS Moriwaki
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dorian WinterDieter Maurer
Sep 26, 2013·Journal of Translational Medicine·Nathalie AccartRonald Rooke
Jul 13, 2000·Seminars in Cutaneous Medicine and Surgery·J M MucheW Sterry
May 1, 2004·Dermatology : International Journal for Clinical and Investigative Dermatology·Taku FujimuraHachiro Tagami

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