Tumour-type-specific capillary endothelial cell stainability in malignant B-cell lymphomas using antibodies against CD31, CD34 and Factor VIII

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
K Norrby, Börje Ridell

Abstract

The microvessel density (MVD) was assessed in lymph nodes infiltrated by diffuse large B-cell lymphomas, mantle cell lymphomas, chronic lymphatic leukemia and follicular lymphomas, and in lymphadenitis. Serial sections of formalin-fixed and paraffin-embedded tissue were stained with antibodies against CD31, CD34 or Factor VIII. Using light microscopy and computerised image analysis, the number and size of individual immunostained vessel profiles within a preselected area size range corresponding to capillaries, postcapillary venules, small collecting venules and small arterioles were determined. A significantly larger number of vessels were registered following staining with anti-CD34 than with anti-CD31 or anti-Factor VIII. Moreover, among the smallest capillary-sized vessel profiles in all lesion types, there was a selective relative loss of stainability of anti-CD31 and anti-Factor VIII, resulting in a substantial total loss of visualised capillary-sized vessels compared with anti-CD34. In fact, the number of non-detected capillaries following staining with anti-CD31 and anti-Factor VIII was significantly tumour type specific. These findings influence how we evaluate MVD data in B-cell lymphomas and possibly also other tumou...Continue Reading

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Citations

Mar 30, 2004·Gynecologic Oncology·Sabas Carlos VieiraNatália Guimarães De Moraes
May 16, 2012·Journal of Pediatric Orthopedics·Pietro DalmonteGianmichele Magnano
Feb 26, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Darko AnticVladan Cokic
Jun 19, 2008·Blood·Sergey V NovitskiyMikhail M Dikov
Jun 22, 2014·Annales de dermatologie et de vénéréologie·A de VitryJ-L Schmutz
Mar 21, 2006·Acta Oncologica·Per AlbertssonKlas Norrby
Oct 11, 2008·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·D WangG P Siegal
Jul 16, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Minna TaskinenSirpa Leppä
Jan 10, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L Smolej, P Kasparová
Jul 11, 2008·Microscopy Research and Technique·Zbynĕk TonarBirgitt Wolfesberger
Sep 23, 2006·Journal of Cellular and Molecular Medicine·K Norrby
Nov 14, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Dita GratzingerYasodha Natkunam

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