Tunable Composition of Dynamic Non-Viral Vectors over the DNA Polyplex Formation and Nucleic Acid Transfection

Polymers
Lilia ClimaMariana Pinteala

Abstract

Polyethylene glycol (PEG) functionalization of non-viral vectors represents a powerful tool through the formation of an overall surface charge shielding ability, which is fundamental for efficient nucleic acid delivery systems. The degree of non-viral vector PEGylation and the molecular weight of utilized PEG is crucial since the excessive use of PEG units may lead to a considerable reduction of the DNA-binding capacity and, subsequently, in a reduction of in vitro transfection efficiency. Herein, we report a detailed study on a series of dynamic combinatorial frameworks (DCFs) containing PEGylated squalene, poly-(ethyleneglycol)-bis(3-aminopropyl) of different lengths, and branched low molecular weight polyethylenimine components, reversibly connected in hyperbranched structures, as efficient dynamic non-viral vectors. The obtained frameworks were capable of forming distinct supramolecular amphiphilic architectures, shown by transmission electron microscopy (TEM) and dynamic light scattering (DLS), with sizes and stability depending on the length of PEG units. The interaction of PEGylated DCFs with nucleic acids was investigated by agarose gel retardation assay and atomic force microscopy (AFM), while their transfection effici...Continue Reading

References

Mar 14, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·W ZaunerA M Haines
Apr 4, 2003·Biological & Pharmaceutical Bulletin·Shi-Joon SungJung-Ki Park
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Abdelatif Elouahabi, Jean-Marie Ruysschaert
Jul 10, 2010·Nature Reviews. Drug Discovery·Robby A Petros, Joseph M DeSimone
Aug 16, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Didier DesmaëlePatrick Couvreur
Dec 16, 2011·Journal of Advanced Pharmaceutical Technology & Research·Vishal R Patel, Y K Agrawal
Aug 14, 2012·Chemical Society Reviews·Sangram Keshari SamalPeter Dubruel
Nov 14, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Elise LepeltierPatrick Couvreur
Feb 25, 2014·Biochimica Et Biophysica Acta·Nathan R Rose, Robert J Klose
Mar 19, 2014·Biochimica Et Biophysica Acta·Scott B Rothbart, Brian D Strahl
Jul 6, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Atul KolateAmbikanandan Misra
Dec 17, 2014·Chemical Communications : Chem Comm·Romina CatanaMariana Pinteala
Jan 15, 2015·Angewandte Chemie·Jean-Marie Lehn
Jul 15, 2015·Organic & Biomolecular Chemistry·Ioana-Andreea Turin-MoleavinMihail Barboiu
Jul 17, 2015·Chemical Reviews·Yan Zhang, Mihail Barboiu
Oct 13, 2015·Advanced Drug Delivery Reviews·Jung Soo SukLaura M Ensign
Oct 16, 2015·Chemical Communications : Chem Comm·Lilia ClimaMihail Barboiu
Jun 15, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sourav Bhattacharjee
Nov 9, 2017·International Journal of Biological Macromolecules·Matteo CiocciSonia Melino
Jul 31, 2018·Advanced Drug Delivery Reviews·Andreas Bernkop-Schnürch
Mar 28, 2019·Biomaterials Research·Y K Sung, S W Kim
Apr 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bogdan Florin CraciunMariana Pinteala

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Citations

Jul 31, 2020·Materials·Mariana PintealaRadu D Rusu
Oct 14, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dandan SuSébastien Ulrich
Aug 5, 2021·Angewandte Chemie·Andrei DiaconuStéphane P Vincent

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Methods Mentioned

BETA
transfection
AFM
Assay
electrophoretic light scattering
electrophoresis
atomic force microscopy

Software Mentioned

GraphPad
Delsa Nano
MestReNova
GraphPad Prism
ImageJ
Mestrelab

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