Tunable DNA Hybridization Enables Spatially and Temporally Controlled Surface-Anchoring of Biomolecular Cargo.

Langmuir : the ACS Journal of Surfaces and Colloids
Roland HagerStefan Howorka

Abstract

The controlled immobilization of biomolecules onto surfaces is relevant in biosensing and cell biological research. Spatial control is achieved by surface-tethering molecules in micro- or nanoscale patterns. Yet, there is an increasing demand for temporal control over how long biomolecular cargo stays immobilized until released into the medium. Here, we present a DNA hybridization-based approach to reversibly anchor biomolecular cargo onto micropatterned surfaces. Cargo is linked to a DNA oligonucleotide that hybridizes to a sequence-complementary, surface-tethered strand. The cargo is released from the substrate by the addition of an oligonucleotide that disrupts the duplex interaction via toehold-mediated strand displacement. The unbound tether strand can be reloaded. The generic strategy is implemented with small-molecule or protein cargo, varying DNA sequences, and multiple surface patterning routes. The approach may be used as a tool in biological research to switch membrane proteins from a locally fixed to a free state, or in biosensing to shed biomolecular receptors to regenerate the sensor surface.

References

Nov 19, 2005·Science·Kaspar D MossmanMichael L Dustin
May 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Keyue ShenLance C Kam
Nov 11, 2008·Nature Methods·Michaela SchwarzenbacherGerhard J Schütz
Nov 13, 2008·Annual Review of Physical Chemistry·Wesley R Browne, Ben L Feringa
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Benjamin GeigerAlexander D Bershadsky
Feb 12, 2009·Nano Letters·Jinghuan HuangJoachim P Spatz
Aug 13, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Zachary R TaylorDavid W Schmidtke
Nov 10, 2009·Journal of the American Chemical Society·David Yu Zhang, Erik Winfree
Nov 27, 2009·Nature·Matthias P LutolfHelen M Blau
Oct 30, 2010·Angewandte Chemie·Barbara SaccàChristof M Niemeyer
Jan 1, 2007·Nanoscale Research Letters·Paula M MendesJon A Preece
Sep 17, 2011·Journal of the American Chemical Society·Michael ErkelenzChristof M Niemeyer
Feb 19, 2014·Current Opinion in Chemical Biology·Rebecca MeyerChristof M Niemeyer
Mar 22, 2014·Soft Matter·Stefan Howorka, Jan Hesse
Nov 11, 2014·Nature Communications·Robert R F MachinekAndrew J Turberfield
Apr 22, 2015·Nature Communications·Eva SevcsikGerhard J Schütz
Jul 27, 2015·Biosensors & Bioelectronics·Anna-Maria LippThomas Haselgrübler
Dec 8, 2015·Angewandte Chemie·Alessandro AngelinChristof M Niemeyer
Jul 9, 2016·Scientific Reports·Katherine E DunnAndy M Tyrrell
Apr 18, 2018·Nature Immunology·Mario BrameshuberJohannes B Huppa
May 19, 2018·Biomolecules·Gergő FülöpEva Sevcsik
Jun 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Anand Lopez, Juewen Liu
Jan 23, 2007·Soft Matter·Sami Alom Ruiz, Christopher S Chen

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Citations

Nov 23, 2021·Journal of the American Chemical Society·Yuliia VybornaAndré Estevez-Torres

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Methods Mentioned

BETA
biosensing
atomic force microscopy
AFM
fluorescence microscopy
electrophoresis
acetylation
Atomic

Software Mentioned

ImageJ
Gwyddion

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