Tunable Enzymatically Cross-Linked Silk Fibroin Tubular Conduits for Guided Tissue Regeneration

Advanced Healthcare Materials
Cristiana R CarvalhoJoaquim M Oliveira

Abstract

Hollow tubular conduits (TCs) with tunable architecture and biological properties are in great need for modulating cell functions and drug delivery in guided tissue regeneration. Here, a new methodology to produce enzymatically cross-linked silk fibroin TCs is described, which takes advantage of the tyrosine groups present in silk structure that are known to allow the formation of a covalently cross-linked hydrogel. Three different processing methods are used as a final step to modulate the properties of the silk-based TCs. This approach allows to virtually adjust any characteristic of the final TCs. The final microstructure ranges from a nonporous to a highly porous network, allowing the TCs to be selectively porous to 4 kDa molecules, but not to human skin fibroblasts. Mechanical properties are dependent both on the processing method and thickness of the TCs. Bioactivity is observed after 30 days of immersion in simulated body fluid only for the TCs submitted to a drying processing method (50 °C). The in vivo study performed in mice demonstrates the good biocompatibility of the TCs. The enzymatically cross-linked silk fibroin TCs are versatile and have adjustable characteristics that can be exploited in a variety of biomedica...Continue Reading

References

Sep 1, 1990·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B L RydevikS R Garfin
Jan 1, 1996·Reviews in the Neurosciences·V B DoolabhS E Mackinnon
May 24, 2001·Journal of Biomedical Materials Research·S Yoshii, M Oka
Nov 7, 2002·Biomaterials·Mingzhong LiNorihiko Minoura
Dec 29, 2004·Biomaterials·Rebecca L HoranGregory H Altman
Feb 22, 2005·Biomaterials·Thomas FreierMolly S Shoichet
Feb 2, 2006·Biomaterials·Tadashi Kokubo, Hiroaki Takadama
Jul 24, 2007·Biomaterials·Lorenz UebersaxLorenz Meinel
Aug 31, 2007·Biomaterials·Michael LovettDavid L Kaplan
Aug 5, 2008·Tissue Engineering. Part B, Reviews·Franklin Rivera Bueno, Sameer B Shah
Jan 2, 2010·Biomaterials·Keiji NumataDavid L Kaplan
May 15, 2010·Acta Biomaterialia·Benedetto MarelliMaria Cristina Tanzi
Jun 16, 2010·Biomaterials·Sang-Hyug ParkDavid L Kaplan
Jul 31, 2010·Science·Fiorenzo G Omenetto, David L Kaplan
Jan 12, 2011·Organogenesis·Michael LovettDavid L Kaplan
Jan 26, 2011·Annals of Plastic Surgery·Amir Mahan GhaznaviKacey G Marra
Aug 5, 2011·Tissue Engineering. Part B, Reviews·Alexander R NectowDavid L Kaplan
Dec 22, 2011·Connective Tissue Research·Lester SmithStavros Thomopoulos
Mar 31, 2012·Journal of Materials Science. Materials in Medicine·Amir ZareidoostAmir Amanzadeh
Mar 31, 2012·American Journal of Respiratory Cell and Molecular Biology·Xiangwei HuangYong Zhou
Jun 28, 2012·Journal of Neural Engineering·Katelyn E Swindle-ReillyRebecca Kuntz Willits
Nov 10, 2012·Advanced Drug Delivery Reviews·Banani KunduXungai Wang
Jan 11, 2013·Journal of Reconstructive Microsurgery·P Konofaos, J P Ver Halen
May 18, 2013·Journal of Biomedical Materials Research. Part a·Xiaoming LiFumio Watari
Dec 3, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Koji Kojima, Charles A Vacanti
Oct 3, 2014·BioMed Research International·D Grinsell, C P Keating
Feb 24, 2015·Cardiology in Review·Vita N Jaspan, George L Hines
Jun 21, 2015·Progress in Neurobiology·Valeria Chiono, Chiara Tonda-Turo
Dec 19, 2015·Journal of Nanoscience and Nanotechnology·Xiaoqing WangHongli Shan
Dec 31, 2015·Microsurgery·Matthias A ReichenbergerSebastian Fischer
Mar 2, 2016·Biotechnology and Bioengineering·Leyla ZilicJohn W Haycock
Mar 16, 2016·Journal of Tissue Engineering·Basak Acan ClementsJoachim Kohn

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Citations

Oct 24, 2019·Biomaterials Science·Cristiana R CarvalhoJoaquim M Oliveira
Dec 12, 2019·Frontiers in Bioengineering and Biotechnology·Cristiana R CarvalhoRui L Reis
Nov 1, 2020·Biomaterials·Wissam FarhatAlexandra Fuchs
Feb 18, 2021·Biomedical Materials·Maryam FarokhiHeinz Redl
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Mathieu GoczkowskiVéronique Larreta-Garde
May 27, 2021·Advanced Healthcare Materials·Qingqing LuDavid L Kaplan
Aug 14, 2021·Acta Biomaterialia·Prerak Gupta, Biman B Mandal
Jan 19, 2021·ACS Biomaterials Science & Engineering·Renchuan YouMingzhong Li
Jul 5, 2021·Advanced Healthcare Materials·Chunliang ZhangXingyu Jiang

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