PMID: 11924703Apr 2, 2002Paper

TUNEL: a useful screening method in sudden cardiac death

International Journal of Legal Medicine
Erik EdstonJesper Johnsson

Abstract

The primary objective of this study was to investigate if detection of apoptosis in the heart can be used to diagnose early myocardial ischaemia. The material consisted of myocardial tissue from autopsy cases: 10 cases with occlusive, thrombotic coronary artery disease and acute myocardial infarction, 10 cases of sudden cardiac death without coronary artery disease (CAD) and 8 controls without cardiovascular disease and with known causes of death. Necrotic changes in the myocardium were detected with hematoxylin-erythrosin-saffron, Mallory's PTAH stain and with antibodies against complement 9. Apoptotic nuclei were visualised with two different kits using the terminal deoxynucleotidyl transferase-mediated desoxyuridinetriphosphate nick end-labeling (TUNEL) method on histological sections. In the patients with CAD, early myocardial infarction was found in one defined area of the ventricular wall; apoptotic myocyte nuclei were observed not in the necrotic lesions, but evenly spread usually without a gradient, all over the myocardium with a mean number per high power field of 29% (range 3-56%) of the total number of myocyte nuclei. In the sudden cardiac deaths without CAD, necrosis was scarce and distributed both focally and irreg...Continue Reading

Citations

Oct 18, 2002·Journal of Cellular Physiology·Antonio AbbateAlfonso Baldi
Sep 11, 2010·Critical Reviews in Food Science and Nutrition·S Zahra Bathaie, S Zeinab Mousavi
Mar 30, 2010·Journal of Forensic Sciences·Nick I BatalisKim A Collins
Sep 18, 2004·The American Journal of Cardiology·Ville KytöLiisa-Maria Voipio-Pulkki
Feb 16, 2012·Redox Report : Communications in Free Radical Research·Athanasios Chalkias, Theodoros Xanthos
Oct 18, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Liuyi DongZhiwu Chen
Jul 10, 2016·International Journal of Legal Medicine·Sara SabatassoValentin Djonov
Mar 8, 2005·International Journal of Legal Medicine·Cristoforo PomaraVittorio Fineschi
May 13, 2017·International Journal of Legal Medicine·Sara SabatassoTony Fracasso

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